Wyniki wyszukiwania - BazTech

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Boron-doped nanocrystalline diamond films as substrates for adhesion, growth and differentiation of human osteoblast-like cells

Bacakova L., Grausova L., Kromka A., Vacik J., Lisa V., Rezek B., Haenen K.

Engineering of Biomaterials

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2010

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Vol. 13, no. 96-98

138--139

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Micro- and nanopatterned surfaces for guided adhesion, growth and phenotypic maturation of cells

Bacakova L., Filova E., Grausova L., Vandrovcova M., Parizek M., Novotna K., Svorcik V., Vacik J., Rypacek F., Kromka A., Heitz J., Shard A.

Engineering of Biomaterials

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2009

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Vol. 12, no. 89-91

18--21

EN

Micropatterned surfaces were created by UV light-irradiation of polytetrafluoroethylene through a metallic mask, by successive plasma polymerization of acrylic acid and 1,7-octadiene, or by creation of prominences and grooves by deposition of fullerenes C60 through a metallic mask. All these surface types were capable of inducing regionally-selective adhesion, proliferation and phenotypic maturation of vascular endothelial cells, vascular smooth muscle cells or human bone-derived MG 63 cells. Nanopatterned surfaces created by tethering GRGDSG oligopeptides through polyethylene oxide chains on a polymeric surface promoted spreading, formation of focal adhesion plaques and DNA synthesis in vascular smooth muscle cells. Surfaces nanopatterned with nanocrystalline diamond gave good support for the adhesion, growth and metabolic activity of osteoblast-like MG 63 cells.

3

Growth of cells on carbon coatings manufactured in new MW/RF reactor

Subrtova I., Grausova L., Bacakova L., Kaczorowski W.

Inżynieria Biomateriałów

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2006

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R. 9, nr 56-57

19-21

EN

Due to unique physico-chemical properties and good biocompatibility are carbon layers considered to be promising material for wide field of biomedical applications. In this study, carbon films were manufactured in microwave and radio frequency plasma reactor using dual frequency method (MW/RF PCVD - microwave and ratio frequency plasma chemical deposition). Four various processes of deposition were used for preparation layers on substrates of medical stainless steel AISI 316 L. On samples of all four processes, growth and adhesion of cells were observed.

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Biological effects of polymers modified with carbon nanotubes on human osteoblast-like MG 63 cells

Grausova L., Bacakova L., Frączek A., Błażewicz S., Błażewicz M., Pepka M.

Inżynieria Biomateriałów

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2006

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R. 9, nr 58-60

14-16

EN

The tested materials were represented by a pure terpolymer of polytetrafluorethylene-polyvinyldifluoride-polypropylene (PTFE-PVDF-PP), pure polysulphone (PSU) and PSU modified with single- or multiwalled carbon nanotubes in concentrations of 0.5, 1 or 2 wt%. As control samples, a polystyrene cell culture dish and microscopic glass coverslips were used. The number and viability of human osteoblast-like MG 63 cells in cultures on these materials was detected with a Cell Viability Analyzer (Vi-CELL XR, Beckman Coulter) on 1, 3 and 7 days after seeding. On all tested samples, the cell number was similar or lower than that detected on the control polystyrene dishes. The cell viability on day 1 after seeding was relatively low on PTFE-PVDF-PP and some nanotube-containing samples, ranging from 10 to 100% of living cells, but on day 7 after seeding, it reached at least 90% on all tested samples. The cell spreading area was detected in cells after immunocytochemical staining of beta-actin on day 3 after seeding. In nanotube-containing samples, especially those with multi-walled nanotubes, this area was similar or even larger than that on the control materials. The beta-actin cytoskeleton was well developed in cells on all nanotube-containing materials and similar to that in cells on control surfaces. Thus, it can be concluded that nanotube-containing PSU supports the adhesion and growth of osteoblast-like cells and could be used for construction of bone implants in which the anchorage in the surrounding bone tissue is desirable.

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Human osteoblast-like cells MG 63 in cultures on polymer-carbon-alginate composites

Grausova L., Bacakova L., Stodolak E., Blazewicz S., Mikołajczyk S.

Inżynieria Biomateriałów

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2005

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R. 8, nr 47-53

6--9

EN

Adhesion and growth of human osteoblast-like MG 63 cells (seeding density of 17964 cells/cm2) was studied in cultures on the following 7 groups of artificial materials developed for bone tissue engineering: terpolymer of polypropylene, polytetrafluorethylene and polyvinyldlfluoride (CPO), terpolymer reinforced wlth carbon fibres (CP4) or carbon fabric (CP5), terpolymer reinforced with carbon fibres and with pores created by addition and dissolution of alginate fibres (CP6) or powder (CP7), terpolymer reinforced with carbon fibres and containing the alginate powder (CP8) or fibres (CP9). On day 1 and 3 after seeding, the numbers of MG63 cells on all tested materials was similar to the values obtained on the control polystyrene culture dish (PS). However, on day 7, the cell number, ranging from 17766±3180 to 67002±6850 cells/cm2, increased in the following order: CP0

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Adhesion of bone and vascular cells on carbon fibre-reinforced carbon composites coated with a fullerene layer

Bacakova L., Grausova L., Vacik J., Jungova I.

Inżynieria Biomateriałów

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2005

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R. 8, nr 47-53

3--6

EN

Carbon fibre-reinforced carbon composites (CFRC), i.e. materials promising for hard tissue surgery, were coated by a fullerene layer in order to strengthen the material surface and create its nanostructure pattern which is known to be attractive for colonization with bone cells. The fullerene layer was relatively resistant to wear, at least swabbing with cotton, rinsing with liquids and exposure to cells and proteolytic enzymes. Both human osteoblast-like MG 63 cells and rat vascular smooth muscle cells (VSMC) in 1-and 2-day-old cultures adhered to these surfaces in Iower numbers in comparison with the control uncoated material and tissue culture polystyrene. In addition, the VSMC on the fullerene-coated surfaces were less spread. The Iower cell adhesion was probably due to a relatively high hydrophobicity of fullerenes. On the other hand, the spreading of MG 63 cells was comparable to that observed on the control surfaces, and these cells also assembled dot-like vinculin-containing focal adhesion plaques and relatively rich fine filamentous beta-actin cytoskeleton. We suppose that the cell adhesion may be enhanced by derivatization of fullerenes with specific chemical functional groups or peptidic ligands for cell adhesion receptors.