Wyniki wyszukiwania - BazTech

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Adsorbenty ze związanymi cieczami jonowymi i ich wykorzystanie w przygotowaniu próbek oligonukleotydów

Nuckowski Łukasz, Zalesińska Ewa, Studzińska Sylwia

Wiadomości Chemiczne

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2020

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[Z] 74, 7-8

545--565

EN

Oligonucleotides are short fragments of nucleic acids. They have a growing potential in medicine, especially as diagnostic and therapeutic agents. In most cases, these compounds are determined in the complex biological matrix. Thus, the sample preparation step is very important in their bioanalysis. Solid-phase extraction is a predominant technique in this field. However, presently used for this purpose adsorbents have disadvantages. They ensure low extraction effectiveness and procedures using them are labor-intensive or time-consuming. Ionic liquids, since their discovery, are objects of intensive interest of scientists. Their scientific attractiveness is connected with their unique properties. They are used in separation and sample preparation techniques, such as liquid-liquid extraction using water-immiscible ionic liquids. This approach was also used in the extraction of oligonucleotides. Adsorbents modified with ionic-liquids have growing potential in extraction techniques. Few types of materials are used, namely carbon, polymers, and silica. A common feature of these materials modified with ionic liquids is the ion exchange character. Nonetheless, carbon nanomaterials are coated or covalently modified with ionic liquids, and they are used mainly for nonpolar compounds. Polymer and silica-based adsorbents are used mainly for acidic compounds. Polymers are characterized by the highest stability of the presented materials. Due to their ion-exchange properties crosslinked poly(ionic liquids) were used also for extraction of unmodified and modified oligonucleotides. The optimized procedure applying the material with bonded zwitterion ionic liquid gives high recoveries. It is concurrent for presently used adsorbents, thus solves problems connected with their usage. Moreover, it can be used for biological samples without any pre-purification.

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Klasyczne i nowe metody przygotowania próbek oligonukleotydów antysensownych przed analizą chromatograficzną

Nuckowski Łukasz, Kilanowska Anna, Studzińska Sylwia

Analityka : nauka i praktyka

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2020

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nr 1

4--11

PL

Chromatograficzna analiza oligonukleotydów antysensownych wymaga przygotowania próbek. Zwykle wiąże się to z czasochłonnymi procedurami lub też koniecznością zastosowania odczynników znacząco wpływających na czułość metod chromatograficznych.

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Analiza oligonukleotydów antysensownych pierwszej i drugiej generacji za pomocą chromatografii par jonowych

Kaczmarkiewicz Anna, Nuckowski Łukasz, Studzińska Sylwia, Buszewski Bogusław

Analityka : nauka i praktyka

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2019

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nr 2

4--11

PL

Rosnące zainteresowanie badaczy potencjalnymi terapeutykami, jakimi są oligonukleotydy antysensowne, sprawia, że wymagana jest ich czuła analiza na różnych etapach badań klinicznych.