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Rapid detection and enumeration of coliforms and Escherichia coli in River Nile using membrane filtration technique

Shash S. M., Kamel M. M., Al-Wasify R. S., Samhan F. A.

Environmental Biotechnology

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2010

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Vol. 6, No. 1

6-10

EN

The purpose of this investigation was to compare Rapid Hicoliform Agar® (RHA, Himedia, India) with the conventional m-Endo Agar (DIFCO, Michigan, USA) for detection and enumeration of Total Coliforms (TC) and with the conventional Seven Hour Fecal Coliforms Agar (7hFC) for detection and enumeration of Escherichia coli using Membrane Filtration (MF) in River Nile water, Egypt. Sixty samples were collected (five samples per month) from five regions (Kafr El-Elw, El-Maasara, El-Giza, Embaba and El-Galatma) during one year (December 2005-November 2006). The results showed that total coliforms counts ranged from 103 to 104 CFU*100ml-1 using RHA but using m-Endo Agar the counts were around 104 CFU*100ml-1. Escherichia coli counts ranged from 103 to 104 CFU*100ml-1 using 7hFC Agar, while using RHA the counts ranged from 103 to 104 CFU*100ml-1. Statistical analysis showed that there was no significant difference (p=0.423) between total coliforms counts using m-Endo Agar and RHA media, also, there was no significant difference (p=0.546) between fecal coliforms (E. coli) counts using 7hFC Agar and RHA media. We conclude that RHA is a potential alternative medium for detection and enumeration of total coliforms and E. coli at the same time and the same Petri dish within 24h. Also, RHA medium was a very sensitive medium, which did not require any confirmatory tests and secured rapid recovery of total coliforms or E. coli.

2

Microbiological criteria of tap water passed through some storage water tanks in Greater Cairo

Osman G. A., Kamel M. M., Al-Herrawy A. Z.

Environmental Biotechnology

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2010

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Vol. 6, No. 2

61-65

EN

Shortage in drinking water supply led to an increase of pumping water to storage tanks and its redistribution to the consumers. The water quality may be changed according to the storage condition and to the deficiency in cleaning of those tanks. Water samples were taken from the tanks of Helwan and El-Giza governorates (Cairo, Egypt) and examined to evaluate microbial water quality. The results revealed that all water samples were free from total coliforms, faecal streptococci, sulphite reducing bacteria (clostridia), salmonellae groups and total vibrios. The total bacterial counts at 37°C and 22°C were 32 to 185 and 11 to 135 cfu*100ml-1, respectively. The total yeast and fungi counts were 9 to 66 and 4 to 35 cfu*100ml-1, respectively. Total counts of staphylococci (from 2 to 17 cfu*100ml-1), Pseudomonas aeruginosa (from 33 to 58 cfu*100ml-1), and coliphage (from 7 to 13 cfu*100ml-1) were also estimated. The free-living amoebae were detected in 75% of the examined storage water tank samples. According to the Egyptian standard for drinking water, most of the examined water samples were not acceptable for human consumption.

3

Some studies on dyeing properties of cotton fabrics with Crocus sativus (saffron) (flowers) using an ultrasonic method

Kamel M. M., Helmy H. M., El Hawary N. S.

Autex Research Journal

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2009

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Vol. 9, no. 1

29--35

EN

Cotton fabrics were dyed with natural colouring matter extracted from Crocus sativus (saffron) using a traditional heating method and a power ultrasonic method. Factors affecting the dyeing properties such as pH values, salt concentration, temperature, duration of dyeing bath, and ultrasonic power were studied. Environmentally friendly mordants were used in pre-mordanting, post-mordanting, and simultaneous-mordanting methods. The fastness properties including light, washing, and perspiration of dyed and mordanted fabrics were assessed. Colour strength (K/S) was measured for dyed and dyed mordanted cotton fabrics.

4

Some studies on dyeing properties of cotton fabrics with curcuma longa (turmeric) (roots) using ultrasonic method

Kamel M. M., El-Hossamy M., Helmy H. M., El-Hawary N. S.

Polish Journal of Applied Chemistry

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2008

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nr 3-4/07,1-2/08

101-121

EN

Cotton fabrics were dyed with natural coloring matter extracted from curcuma longa (turmeric) using traditional heating method and power ultrasonic method. Factors affecting dyeing properties such as pH values, salt conc., temperature, time of dyeing bath and ultrasonic power were studied. Environmentally friendly mordants were used through pre-mordanting, post mordanting, simultaneous mordanting methods. The fastness properties including light, washing, and perspiration for dyed and mordanted fabrics were assessed. Color strength (K/S)& color data CIE lab (L*, a*, b*) were measured for dyed cotton fabrics before and after dyeing and mordanted cotton fabric.