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HPLC analysis and detection of l-deprenyl

Csomor V., Laufer R., Pöstényi Z., Kalász H., Adeghate E., Nurulain S. M., Tekes K., Adem A.

Acta Chromatographica

2014

Vol. 26, no. 4

649--656

l-Deprenyl and its major metabolites were subjected to reversed-phase high-performance liquid chromatography (HPLC). The separation was monitored using both ultraviolet (UV) and electrochemical detectors. Ultraviolet absorbance detected l-deprenyl, l-nordeprenyl, l-methamphetamine, and l-amphetamine. Amperometric detection was specific and sensitive to the parent compound (l-deprenyl, a tertiary amine) only. Peaks of the major L-deprenyl metabolites did not give any comparable signal using amperometric monitoring.