In this work, three-phase partitioning (TPP) was used for the purification of laccase from liquid cultures of Trametes versicolor. For determining the optimal conditions of TPP process, parameters such as initial pH (6.5, 7.0, 7.5, 8.0), ammonium sulphate saturation (20%-80%) and the water phase to tert-butanol ratio (1:0.5, 1:1, 1:2), were analyzed. The best conditions with 73% recovery and 24-fold purification was obtained with the use of 50% saturation with ammonium sulphate, water phase to tert-butanol ratio of 1:1 and initial pH 7.0. The molecular mass of the purified laccase secreted by analyzed strain T. versicolor was found for ≈ 66 kDa. The results showed that TPP is an efficient method for the fractionation and purification of laccase obtained from liquid cultures of T. versicolor and it allows for obtaining the relatively pure enzyme without the use of time-consuming and costly chromatographic methods.
The main practical problem in the Logistics Department at the company is overstaffing in the unit responsible for the flow of materials. Carried out by the authors of the article, some detailed research on the process analysis in the discussed company indicates that the main cause of such a situation is related to an increase in the amount of transported material and a lack of redesigning the material flow in spite of the increased levels of production and acquisition of new projects. At present, at the company the material is prepared and transported by eleven employees whose work is not standardized and impossible to control. Such a large number of people working at the same time at the warehouse and moving through the transport corridors seriously affects the level of safety in a negative way. Therefore, the authors of the publication have suggested three solutions which involve some logistic changes in the existing process in order to increase safety and to reduce the number of staff involved in the transport.
The aim of the article is to present a concept related to the improvement of the logistics process at the company, by the modernization of the main railway siding of the terminal. The paper present the reasons for the decision that was once made in order to obtain the expected advantages after the modernization. The comparison of the incurred expenses and the profits that might be gained in the future allows us to state that the decision about the modernization was the right one. The decision about the modernization also referred to a trend towards an increase in cargo handling operations, which could be observed at the company at that time. The decision about modernization was made when the terminal operated using its full capacities of the railway siding. Therefore, the modernization was necessary in order to meet expectations expressed by the current and potential customers who might appear in the future.
The article aims at the presentation of a concept referring to the improvement of warehouse management in the X production plant. The authors suggest two solutions: using storage racks at the picking process site and warehouse automation. Ultimately, the authors of the article present their basic calculation of the costs, indicating the increase in efficiency after the implementation of the discussed improvements.
5
Dostęp do pełnego tekstu na zewnętrznej witrynie WWW
Celem artykułu jest przedstawienie koncepcji usprawnienia procesu spawania sekcji przestrzennych w kadłubie statku. Przedmiotem analizy projektu będzie styk międzyblokowy, który pospawany zostanie dwoma różnymi metodami. W artykule przedstawione zostaną rozwiązania techniczne wspomagające procesy spawalnicze oraz pomiary i obliczenia umożliwiające porównanie obu metod spawania.
EN
The aim of the article is to present the concept of improving the welding process in the hull of the ship. The analysis of the project will contact, which will be welded with two different methods. The article presents technical support welding processes as well as measurements and calculations allowing comparison of both welding methods.
W tekście przedstawiono nowe rozwiązania procesu montażu elementów konstrukcyjnych w jednostkach offshore stoczni X. Nowa proponowana metoda, polegająca na zastosowaniu uchwytów magnetycznych, pozwala na skrócenie czasu montażu z trzech godzin do półtorej godziny, tj. o 50%. Ponadto, w metodzie tej obniżone zostaną koszty materiałów oraz energii elektrycznej. Czas wykonania jednostki offshore ulegnie również skróceniu, a jakość poprawi się dzięki precyzyjności montażu usztywnień.
EN
The text presents new solutions for the assembly of shipbuilding components in shipyard offshore X. The proposed new method, consisting in the use of magnetic handles, reduces the installation time from three hours to one and a half hours, ie 50%. In addition, the method will reduce the cost of materials and electricity. The execution time of the offshore unit will also be shortened and the quality will improve thanks to the precision of the stiffeners.
The aim of the present study was to evaluate the impact of a rotating magnetic field (RMF) on cellular and biochemical properties of Gluconacetobacter xylinus during the process of cellulose synthesis by these bacteria. The application of the RMF during bacterial cellulose (BC) production intensified the biochemical processes in G. xylinus as compared to the RMF-unexposed cultures. Moreover, the RMF had a positive impact on the growth of cellulose-producing bacteria. Furthermore, the application of RMF did not increase the number of mutants unable to produce cellulose. In terms of BC production effi cacy, the most favorable properties were found in the setting where RMF generator was switched off for the fi rst 72 h of cultivation and switched on for the further 72 h. The results obtained can be used in subsequent studies concerning the optimization of BC production using different types of magnetic fields including RMF, especially.
Celem artykułu jest przedstawienie koncepcji usprawnienia procesu obróbki wstępnej oraz produkcji dżemu truskawkowego poprzez zakup i wprowadzenie odszypułczarki. Nowa maszyna pozwoliła na likwidację wąskiego gardła w produkcji, dzięki czemu wzrosła dzienna wielkość produkcji.
EN
The article presents approaches to improve the pretreatment and production of strawberry jam by purchasing and introducing stalks removing machines. The new machine has allowed the elimination of the bottleneck in production, allowing increased daily production volume.
The aim of the study was to present an experimental investigation of the influence of the RMF on mixing time. The obtained results suggest that the homogenization time for the tested experimental set-up depending on the frequency of the RMF can be worked out by means of the relationship between the dimensionless mixing time number and the Reynolds number. It was shown that the magnetic field can be applied successfully to mixing liquids.
The aim of the study was to analyze the changes in the parameters of bacterial cultures and bacterial cellulose (BC) synthesized by four reference strains of Gluconacetobacter xylinus during 31-day cultivation in stationary conditions. The study showed that the most visible changes in the analyzed parameters of BC, regardless of the bacterial strain used for their synthesis, were observed in the first 10–14 days of the experiment. It was also revealed, that among parameters showing dependence associated with the particular bacterial strain were the rate and period of BC synthesis, the growth rate of bacteria anchored to the cellulose fibrils, the capacity to absorb water and the water release rate. The results presented in this work may be useful in the selection of optimum culturing conditions and period from the point of view of good efficiency of the cellulose synthesis process.
11
Dostęp do pełnego tekstu na zewnętrznej witrynie WWW
W artykule zaprezentowano koncepcję zwinności przedsiębiorstw stanowiącą podstawę zapewnienia przewagi konkurencyjnej nad biznesowymi rywalami w zmiennych warunkach otoczenia. Podkreślono duże znaczenie wykorzystywanych technologii i systemów informatycznych w efektywnej konfiguracji logistycznych relacji biznesowych w ramach realizowanych łańcuchów dostaw na dynamicznym rynku. Celem artykułu jest omówienie koncepcji zwinności przedsiębiorstw w perspektywie dynamicznych, zmiennych i wysoce konkurencyjnych warunków otoczenia.
EN
The article presents the concept of corporate agility, which forms the basis of assuring competitive advantage over business rivals in a fluctuating environment. High importance of the technology and IT system use in efficient configuration of business relations within supply chains on dynamic markets was also noted. The goal of the article is to discuss concepts of corporate agility on the perspective of dynamic, fluctuating, and highly competitive environment.
W niniejszym opracowaniu zaprezentowano zróżnicowane podejście do logistyki zaopatrzenia w działalności wytwórczej i usługowej. Autorzy na podstawie analizy literatury przedmiotu dokonali identyfikacji kluczowych determinant logistyki zaopatrzenia, a następnie dokonano analizy porównawczej dla obu form działalności. W celu zapewnienia holistycznego ujęcia tematu zaprezentowane rozważania dotyczą ogółu działalności wytwórczych i usługowych bez uwzględniania specyfiki branż.
EN
In this paper the authors presented diverse approach to purchase logistics in production and service business. The authors, based on the analysis of subject literature, identified the key elements of purchase logistics and afterwards analyzed the determining factors in both business activity types. In order to present a holistic view, the paper focuses on general production and service businesses, without taking into account the specifics of any branch of industry.
W niniejszym artykule zostały przedstawione wyniki badań w zakresie eliminacji strat maszyn występujące na linii produkcyjnej w procesach aparaturowych u producenta farb proszkowych przedsiębiorstwa X. Dodatkowo autorzy zaproponowali własne metody organizacyjne , które zasadniczo zmniejszą straty maszyn na linii produkcyjnej i spowodują większą niezawodność parku maszynowego.
EN
The aim of the paper is to present research results in the elimination of losses occurring machines on the production line in the processes of apparatus manufacturer of powder coatings company X. Additionally, the authors have proposed their own methods of organization, which essentially reduce losses machines on the production line and result in greater reliability of the machine park.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.