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Antibiotics resistance in Enterococcus isolates from poultry waste

Cybulska Krystyna, Krzyśko-Łupicka Teresa

Ecological Chemistry and Engineering. S

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2020

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Vol. 27, nr 2

305--316

EN

The aim of the study was to evaluate the drug resistance of Enterococcus faecalis and Enterococcus faecium isolated from different types of poultry waste. The study material consisted of feather samples (duck, turkey, chicken), sludge and centrifuge sediment, originating from three poultry farms. The study was conducted in two stages; isolation and identification of Enterococcus bacteria from the waste and evaluation of their drug resistance using Kirby-Bauer method. Contamination of the poultry waste with Enterococcus isolates included E. faecium species (79 %) and E. faecalis (21 %). The most contaminated were sludge and sediment from the centrifuge as well as chicken feathers, irrespective of the place and time of sampling. Tested isolates showed multiple resistance and similar reaction to all antibiotics used in the study and E. faecalis strain was more resistant. Enterococcus isolates showed the highest resistance to streptogramins, carbapenems, fluoroquinones, aminoglycosides and penicillins, and the lowest for nitrofurantions and phenicols.

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The effect auto-inoculation of the soil on the change in the amount of live microbial biomass

Rygielski Kacper, Cybulska Krystyna

Ecological Chemistry and Engineering. A

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2020

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Vol. 27, nr 1-2

1--10

EN

Out of three light soils of various parameters were isolated bacteria’s, actinomycetes and fungi. Using those, autovaccines were prepared, individually for each of them. Then, in laboratory soil-conditions were given autovaccines and during incubation, samples were being taken, in which the content of biomass of living microorganisms were determined. The largest amount of biomass of living microorganisms was found in the soil from Stuchowo, the lowest in the soil from Swierzno. During the incubation of soils in the laboratory, the amount of biomass of living microorganisms decreased in soils from Stuchowo and Swierzno, while it increased in the soil from Kepica. Bioaugmentation resulted in a statistically significant increase in the amount of biomass of living microorganisms in all soils tested, reaching up to 30 % compared to non-vaccinated soil. The increase was the highest in the soil from Stuchowo and then in the decreasing order in the soils from Kepica and Swierzno.

3

The effect of ash and compost on the content and bioaccumulation of selected heavy metals

Możdżer Ewa, Cybulska Krystyna, Krzyśko-Łupicka Teresa, Meller Edward

Polish Journal of Chemical Technology

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2019

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Vol. 21, nr 2

20--25

EN

Therefore the carried out study aimed at determination of the effect of high-calcium brown coal ash and compost being produced from municipal sewage sludge on the content and bioaccumulation of heavy metals in potato tubers, wheat grains and rapeseeds during a three-year period. Rapeseeds contained most Cd whereas wheat rains less. Potato tubers, wheat grains and rapeseeds contained more Mn, Ni and Zn in the fertilization objects with municipal sewage sludge with or without coal ash and compared to those where calcium carbonate or coal ash had been introduced into the soil at a dose corresponding to 1.5 Mg CaO · ha−1 at the beginning of this study. Differences in the Mn, Ni and Zn contents in test plants between the fertilization objects with sewage sludge of with and without addition of ash were not significant.