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Wpływ modyfikatora fazy ruchomej na selektywność rozdzielenia w odwróconym układzie faz wysokosprawnej chromatografii cieczowej

Misiołek B., Klimek-Turek A., Dzido T. H.

Wiadomości Chemiczne

2013

[Z] 67, 1-2

133--159

High performance liquid chromatography (HPLC) is an instrumental analytical technique, which is widely used for a separation and determination of a mixture of components in many samples (e.g. of biomedical, pharmaceutical, food, and environmental origin). Despite several decades of the development of this technique, some aspects of the chromatographic process are still open to questions. This is particularly related to mechanisms of retention and selectivity of a separation. Improvement of the separation selectivity can be achieved by a change of the stationary phase type and qualitative and/or quantitative composition of the mobile phase. The replacement of the stationary phase does not ensure a smooth change of selectivity and retention, however, it generates additional costs of analysis. Therefore, the optimal conditions of chromatographic separation can be easily obtained by the change of a composition of the mobile phase, i.e. the type and/or concentration of its modifier (organic solvent). This paper presents an overview of approaches to explanation and interpretation of an influence of mobile phase composition on the retention and separation selectivity in liquid chromatography systems with particular emphasis on modifier type of eluent in the reversed phase high performance liquid chromatography (RP HPLC).