Wyniki wyszukiwania - BazTech

1

Development and validation of gel-chromatographic and spectrophotometric methods for quantitative analysis of bioactive copper complexes in new antihypocupremical formulations

Savic I, Nikolic G, Savic I, Cakic M

Acta Chromatographica

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2010

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Vol. 22, no. 3

375--390

EN

Gel-permeation chromatographic (GPC) and visible spectrophotometric methods have been developed and validated for quantitative analysis of complexes of copper(II) with the polysaccharides pullulan and dextran, active pharmaceutical compounds in new antihypocupremical formulations. Linearity, precision, accuracy, specificity, and limits of detection (LOD) and quantification (LOQ) were determined in accordance with ICH Q2(R1) guidelines. GPC was performed isocratically with redistilled water as mobile phase at a flow rate of 1 mL min -1 Visible spectrophotometry was performed in water, using 640 nm for direct assay of the copper(II) complex with pullulan and dextran. The calculated F and t values at the 95% confidence level were less than the theoretical values, showing there were no significant differences between the performance of the methods.

2

Spectrophotometric determination of lisinopril in commercial dosage forms using N-bromosuccinimide and chloranil

Rahman N., Siddiqui M. R., Azmi S. N. H.

Chemia Analityczna

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2007

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Vol. 52, No. 3

465-480

EN

Two simple, fast, sensitive, and economical spectrophotometric methods for quantification of lisinopril in drug formulations have been developed. Method A was based on the reaction between primary amino group of the drug with N-bromosuccinimide in acetone medium giving a yellow-colored product of maximum absorption at 353 nm. Method B was based on the formation of purple-colored charge transfer complex between the drug and chloranil in 1,4-dioxan-acetonitriIe medium. Beer's law was obeyed in the concentration ranges of 10-200 and 24-600 μg mLTwo simple, fast, sensitive, and economical spectrophotometric methods for quantification of lisinopril in drug formulations have been developed. Method A was based on the reaction between primary amino group of the drug with N-bromosuccinimide in acetone medium giving a yellow-colored product of maximum absorption at 353 nm. Method B was based on the formation of purple-colored charge transfer complex between the drug and chloranil in 1,4-dioxan-acetonitriIe medium. Beer's law was obeyed in the concentration ranges of 10-200 and 24-600 ug mL'1 with molar absorptivities of 1.40 x 103 and 7.28 x 102 L-1 mol cm'1 for methods A and B, respectively. Regression analysis yielded the following calibration equations: A = -2.44 x 10-1 + 3.16 x 10-1 C and A = 5.39 x 10-4+ 1.65 x 10-3C for methods A and B, respectively. For both calibration equations correlation coefficients were 0.9999. Intra- and inter-day precisions were lower than 1.60%. The optimum experimental conditions for the proposed methods have been investigated. Methods A and B have been successfully applied to the analysis of lisinopril in drug formulations. The results have been statistically compared to these obtained applying the British pharmacopoeia method with molar absorptivities of 1.40 x 103 and 7.28 x 102 L-1 mol cm-1 for methods A and B, respectively. Regression analysis yielded the following calibration equations: A = -2.44 x 10-1 + 3.16 x 10-1 C and A = 5.39 x 10-4+ 1.65 x 10-3C for methods A and B, respectively. For both calibration equations correlation coefficients were 0.9999. Intra- and inter-day precisions were lower than 1.60%. The optimum experimental conditions for the proposed methods have been investigated. Methods A and B have been successfully applied to the analysis of lisinopril in drug formulations. The results have been statistically compared to these obtained applying the British pharmacopoeia method

PL

Opracowano dwie proste, szybkie, czule i ekonomiczne spektrofotometryczne metody ilościowego oznaczania lizynoprilu w preparatach farmaceutycznych. Metoda A jest oparta na reakcji picrwszorzędowej grupy aminowej leku z N-bromosukcynimidem w acetonie, w wyniku której powstaje żółty produkt wykazujący maksimum absorpcji przy df. fali 353 nm. Metoda B polega na tworzeniu purpurowego kompleksu z przeniesieniem iadunku miedzy lekiem a chloranilem w mieszaninie 1,4-dioksanu i acetonu. Prawo Beer'a było . spełnione w zakresie stężeń 10-200 i 24-600 1.40 x 103; absorpcja molowa wynosiła 1.40 x 103 and 7.28 x 102 L-1 mol cm-1, odpowiednio w przypadku metody A i B, W wyniku analizy regresji otrzymano dwa równania kalibracyjne A =-2,44 x 10-4 + 3,16 x 10-3 C oraz A = 5,39 x10-4 + 1,65 x 10-3 C, odpowiednio dla metody A i B. Obie metody zoptymalizowano i zastosowano z powodzeniem do analizy lizynoprilu w preparatach farmaceutycznych. Otrzymane wyniki porównano z wynikami uzyskanymi według Farmakopei Brytyjskiej.

3

New spectrophotometric method for determination of cefpodoxime protexil

Srinivasa Rao Y., Chowdary K. P. R., Seshagiri Rao J. V. L. N.

Chemia Analityczna

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2004

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Vol. 49, No. 1

111-116

EN

A simple spectrophotometric method for the determination of pure cefpodoxime proxetil and in its pharmaceutical formulations has been developed. It utilises the pink colour formation following the complexation of cefpodoxime proxetil with sodium metaperiodate and brucine. The maximum absorbance of the complex appears at 520 nm and the system obeys Beerís law in the concentration range: 5-30 mg mL-1. The proposed method is selective, simple and inexpensive for the quantitative determination of cefpodoxime proxetil. It has been statistically validated.

PL

Opracowano prostą metodę spektrofotometrycznego oznaczania cefpodoksymu w stanie wolnym i w postaciach leku. Metoda wykorzystuje powstający różowy kompleks cefpodoksymu z metanadjodancm sodu i bmcyną. Ten chromogen, posiadający maksimum absorpcji przy df. fali 520 nm, spełnia prawo Beera w zakresie stężeń 5-30 ugmL(-1). Metoda została zwalidowana statystycznie. Zaproponowana metoda pozwala na selektywne, proste i ekonomiczne, ilościowe oznaczanie cefpodoksymu.