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1 2011

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Comparison of different columns in analysis of C1–C5 aliphatic acids mixture in ion exclusion chromatography and vacancy ion exclusion chromatography modes

Zapała W., Kostka J, Kaczmarski K.

Acta Chromatographica

2011

Vol. 23, no. 3

377--388

Ion exclusion chromatography (IEC) is a chromatographic technique based on the exclusion of dissociated sample molecules. This method has many applications in various analytical separations of weak acids. An alternative technique is the vacancy IEC (v-IEC), where the column is equilibrated with the sample solution flowing as mobile phase through the system, whereas pure water is injected as a sample. In this case, characteristic negative “vacant” peaks are obtained. The aim of this paper is to compare the efficiency of four RP-HPLC columns in the separation of C1–C5 aliphatic acids mixture by use of classical HPLC and very high pressure liquid chromatography. The investigations were conducted in IEC and v-IEC modes.