Wyniki wyszukiwania - BazTech

1

Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization

Parizek M., Kasalkova N., Bacakova L., Kolarova K., Lisa V., Svorcik V.

Engineering of Biomaterials

|

2009

|

Vol. 12, no. 89-91

25--28

EN

Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.

2

Vascular smooth muscle cells in cultures on biofunctionalized cellulose-based scaffolds

Novotna K., Bacakova L., Lisa V., Havelka P., Sopuch T., Klepetar J.

Engineering of Biomaterials

|

2009

|

Vol. 12, no. 89-91

21--24

EN

Viscose, dialdehyde cellulose and oxidized 6-car-boxycellulose with 2.1 or 6.6wt.% of –COOH groups were prepared. The materials were subsequently functionalized with arginine or chitosan. Both unmodified and biofunctionalized materials were seeded with vascular smooth muscle cells. The morphology of the adhered cells indicated that oxidized 6-carbo-xycellulose with 2.1% content of –COOH groups was the most appropriate of all tested materials for potential use in tissue engineering. The shape of the cells on this material was elongated, which demonstrates adequate adhesion and viability of the cells, while the morphology of the cells on other tested materials was spherical. Moreover, the stability of 6-carboxycellulo-se with 2.1wt.% of –COOH groups in the cell culture environment was optimal, with a tendency to degrade slowly with time. The highest stability was found on the viscose samples, whereas there was very low stability on oxidized 6-carboxycellulose with 6.6 wt. % of –COOH groups, and also on dialdehyde cellulose. Functionalization with arginine or chitosan increased the number of adhered cells on the materials, but not markedly. We did not obtain a significant elevation of the cell population densities with time on the tested samples. These results suggest the possibility of using a cellulose-based material in such tissue engineering applications, where high proliferation activity of cells is not convenient, e.g. reconstruction of the smooth mu-scle cell layer in bioartificial vascular replacements.

3

Micro- and nanopatterned surfaces for guided adhesion, growth and phenotypic maturation of cells

Bacakova L., Filova E., Grausova L., Vandrovcova M., Parizek M., Novotna K., Svorcik V., Vacik J., Rypacek F., Kromka A., Heitz J., Shard A.

Engineering of Biomaterials

|

2009

|

Vol. 12, no. 89-91

18--21

EN

Micropatterned surfaces were created by UV light-irradiation of polytetrafluoroethylene through a metallic mask, by successive plasma polymerization of acrylic acid and 1,7-octadiene, or by creation of prominences and grooves by deposition of fullerenes C60 through a metallic mask. All these surface types were capable of inducing regionally-selective adhesion, proliferation and phenotypic maturation of vascular endothelial cells, vascular smooth muscle cells or human bone-derived MG 63 cells. Nanopatterned surfaces created by tethering GRGDSG oligopeptides through polyethylene oxide chains on a polymeric surface promoted spreading, formation of focal adhesion plaques and DNA synthesis in vascular smooth muscle cells. Surfaces nanopatterned with nanocrystalline diamond gave good support for the adhesion, growth and metabolic activity of osteoblast-like MG 63 cells.

4

Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge

Parizek M., Kasalkova N., Bacakova L., Kolarova K., Lisa V., Svorcik V.

Engineering of Biomaterials

|

2007

|

Vol. 10, no. 67-68

1-4

EN

The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.

5

Vascular smooth muscle cells in cultures on synthetic polymers with adhesive microdomains

Parizek M., Bacakova L., Lisa V., Kubova O., Svorcik V., Heitz J.

Inżynieria Biomateriałów

|

2006

|

R. 9, nr 58-60

7-10

EN

Polyethylene terephtalate was modified by UV light irradiation produced by a Xe2-excimer lamp for 10, 20 or 30 min in an acetylene atmosphere. For creation of microdomains for selective cell adhesion, a contact nickel mask (apertures of the diameter of 500 m, centre-to-centre distance 2 mm) was used. The material was then seeded with rat aortic smooth muscle cells (passage 3, 17 000 cells/cm square). After 1, 3 and 7 days of cultivation, the cells were homogeneously distributed on the samples without any preference of the irradiated microdomains. Moreover, on day 1, the number of initially adhered cells was similar on all tested samples. However, on day 3, the number of cells on the irradiated samples was significantly higher than that on control unmodified PET and increased proportionally to the time of exposure to UV light. On day 7 after seeding, however, the cell number on the unmodified PET exceeded the value on all irradiated samples. In the second set of experiments, polyethylene (PE) was irradiated by Ar+ ions in order to create the adhesive microdomains (dose 10\12-10\14 ions/cm square, energy 150 and 15 keV, contact mask with holes of 100 m diameter and distance 200 m). The highest selectivity of the adhesion and growth of rat aortic smooth muscle cells (89% of all cells) was found on the microdomains created at the energy of 150 keV and the dose of 3x10\12 ions Ar+. The lowest selectivity (30%) occurred on samples irradiated with 150 keV Ar+ ions of the dose of 3x10\14 ions/cm square. Therefore, both methods seem to be suitable for modification of materials with highly hydrophobic surfaces in purpose to increase the cell colonization, for example when constructing bioartificial vascular replacements. The second method can be also used for the creation of domains for a regionally selective adhesion and growth of cells on biomaterials.

6

Adhesion and proliferation of vascular smooth muscle cells on polylactide-polyethylene oxide copolymers with different content and length of polyethylene oxide chains

Filova E., Bacakova L., Lisa V., Kubies D., Machova L., Lapcikova M., Rypacek F.

Inżynieria Biomateriałów

|

2004

|

R. 7, nr 38-42

19-21

EN

We evaluated antiadhesive effects of polymer surfaces prepared from PDLLA-PEO copolymers using PEO with a different molecular weight and different PEO content in comparison with the native poly(L-lactide) (PLLA) surface. All PDLLA-PEO copolymers significantly decreased number of initially adhered cells (by 23-55% in comparison with pure PLLA) as well as spreading area 24 hours after seeding (by 39-79%). Cell proliferation, estimated by cell number on the 6 day after seeding and bromodeoxyuridine (BrdU) labeling index, was significantly lower on PEO-containing copolymers (by 58-96% and 21-35% respectively) compared to pure PLLA surface. Immunofluorescence staining of vinculin showed that the ability of VSMC to form local adhesion plaques was markedly reduced on surfaces with the highest content of PEO (33 and 44%). Thus, these copolymers are promising for creation of surfaces preventing uncontrolled adsorption of proteins and adhesion of cells. Consecutively, binding of defined ligands for cell adhesion receptors would enable to control cell behaviour on these materials, which could be used for construction of vascular prostheses.