Wyniki wyszukiwania - BazTech

Ograniczanie wyników

1 Acta Chromatographica

1 2014

Znaleziono wyników: 1

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: ultra-performance LC

Sortuj według:

Ogranicz wyniki do:

Simultaneous determination of anthraquinone derivatives in Radix et Rhizoma Rhei-based medicines by ultra-performance LC-ESCI-MS/MS multiple reaction monitoring

Wang G. Y., Shi Y. P.

Acta Chromatographica

2014

Vol. 26, no. 2

229--242

A new method using ultra-performance liquid chromatography (UPLC) in combination with tandem mass spectrometry and a multiple reaction monitoring mode (UPLC-MS/MS-MRM) was developed for simultaneous quantitative determination of anthraquinone derivatives in Radix et Rhizoma Rhei-based medicines. A multi-mode electrospray/chemical ionization (ESCI) and negative ion mode with [M-H]− and its fragments under collision-activated conditions were employed in MS/MS-MRM. The quantitative method was validated and applied to simultaneous determination of anthraquinone derivatives in 21 Radix et Rhizoma Rhei-based medicines. The limits of quantification were in the range of 3.90–9.09 ng mL -1. Average recoveries were between 95.5% and 99.8% with relative standard deviations from 1.8% to 5.3%.