Wyniki wyszukiwania - BazTech

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Application of modified silica gel in the process of trypsin immobilization

Miłek Justyna, Kwiatkowska-Marks Sylwia, Trawczyńska Ilona

Technical Sciences / University of Warmia and Mazury in Olsztyn

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2019

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nr 22(1)

35--43

EN

The paper presents the use of modified silica gel for the production of immobilized trypsin from bovine pancreas. Silica gel was modified with 3-aminopropyltriethoxysilane, followed by glutaraldehyde. The influence of stirring time on activity of the prepared biocatalyst was determined for individual stages of the modification. Activity of both native and immobilized trypsin was measured using Kunitz method. At the temperature of 55℃ and pH 7.6 native and immobilized trypsin onto modified silica gel indicate optimum activity. The influence of multiple recycling and storage time on activity of immobilized trypsin was tested. After fourteen days of storage at the temperature of 4℃ immobilized trypsin exhibits 75% of its initial activity.

2

Nanostructure of bovine pericardium treated with trypsin

Turek A., Marcinkowski A., Trzebicka B., Cwalina B., Dzierżewicz Z.

Engineering of Biomaterials

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2009

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Vol. 12, no. 89-91

30--32

EN

Various methods of xenogeneic tissues stabilization have been proposed for the purpose of preparing many tissue-derived biomaterials. One of the most important treatments that may lead to obtaining the good-quality tissue biomaterials seems to be decellularization of such tissues. This process may contribute to the reduction of the most frequent failures resulting from the tissues stabilization. The aim of this work was to determine nanostructure of trypsin-treated bovine pericardium, using atomic force microscopy (AFM). The treatment of bovine pericardium with trypsin in EDTA solution resulted in non significant changes in tissue’s morphology. Demonstrated AFM studies of these tissues revealed no failures on the fibers’ surface in the nanoscale. Thus, our results confirm the expec-tation that decellularization may be considered as one of the most promising methods of the allogeneic and xenogeneic tissues stabilization.

3

Wykorzystanie metody alkacymetrycznego miareczkowania do określenia aktywności trypsyny w trzustce wieprzowej

Sierecka J., Masłowska J.

Zeszyty Naukowe. Chemia Spożywcza i Biotechnologia / Politechnika Łódzka

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1998

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Z. 59

77-86

PL

Opracowano ulepszoną metodę opartą na miareczkowaniu alkacymetrycznym określania aktywności trypsyny w wodnych wyciągach próbek trzustki wieprzowej. Jako substrat stosowano 3% roztwór żelatyny zamiast proponowanego wcześniej roztworu 5%. Analiza statystyczna uzyskanych wyników wykazała, że proponowana metoda daje wyniki powtarzalne i dokładne.

EN

Improved method of alkalimetric titration for the determination of a trypsin activity in an alkaline medium is presented. The method was tested on the pig pancreas. The presented modified method is characterized by a relative short analysis time and satisfactory accurancy and it is required no complicated and at great cost apparatures The proposed method may be recommended for each chemical laboratory.