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The effect of traumatic acid on the growth, metabolite content and antioxidant activity in Wolffia arrhiza (L.) Wimm. (Lemnaceae)

Pietryczuk A., Czerpak R.

Oceanological and Hydrobiological Studies

2012

Vol. 41, No. 1

24-34

The research presented here was conducted in order to determine the influence of exogenous traumatic acid (TA) on the growth, metabolism, and antioxidative activity of vascular water plant, Wolffia arrhiza Wimm. The research was concerned with TA influences, in concentrations of 10^-8 M – 10^-4 M, on fresh W. arrhiza, and on primary metabolites, such as monosaccharides, proteins, chlorophylls a and b, and carotenoids. It was determined that TA causes a substantial increase in these metabolites compared to the control, especially at concentrations of 10^-7 – 10^-6 M. Polyacrylamide gel electrophoresis of proteins (SDS-PAGE) was conducted in order to specify in more detail the qualitative changes in proteins, whose synthesis is stimulated by TA. Under the influence of TA W. arrhiza cells saw an induction of de novo synthesis of 3 proteins with molecular weights of 10, 58, and 90 kDa. It was proven that 10^-7 – 10^-6 M concentrations of TA also increase photosynthesis intensity and the activity of antioxidative enzymes (ascorbate peroxidase, catalase, glutathione reductase, and superoxide dismutase). In cells treated with exogenous TA, lipids' peroxidation decreases (expressed as a drop in malonyl dialdehyde) and sulfhydryl (SH) groups in proteins increase. Based on our research, TA plays an important role in the regulation of growth and metabolism in W. arrhiza. Our results also show that TA possibly participates in the activation of antioxidant enzymes and its probable participation in the metabolic responses of lower water plants to oxidative stresses.

The influence of traumatic acid on the growth and metabolite content of the green alga Chlorella vulgaris

Pietryczuk A., Piotrowska A., Czerpak R.

Oceanological and Hydrobiological Studies

2008

Vol. 37, No.1

3-15

The effect of exogenous traumatic acid, (2E)-dodecene-1,12-dioic acid (TA), at concentrations of 10-6 - 10-3 M on the growth and development of the unicellular green alga Chlorella vulgaris was examined over 7 days. TA plays an important role in algal growth and development. Treatment with 10-5 M TA resulted in the greatest increase in cell numbers, by 77%, chlorophyll a content, by 126%, chlorophyll b, by 45%, total carotenoids, by 65%, monosaccharides, by 111%, and water-soluble proteins, by 37%, in relation to the control. After three days of treatment with 10-5 M TA cultures were analysed by SDS-PAGE, which showed the presence of new polypeptides with molecular weights 13-103 kDa.