The exposure of organisms to environmental stressors affects the expression levels of certain 'stress proteins' that play an important role in protein homeostasis and stress tolerance. Examining the protein profiles by SDS-PAGE and Western blotting analysis, mussels Mytilus trossulus were exposed to cadmium, which induced a number of Hsp70 proteins in accordance with the metal concentrations. In immunodetection two commercial monoclonal antibodies were used to monitor this response in gill tissue. It follows that Hsp70, which is typically induced by moderate heat-shock treatment, is in most cases also induced in the presence of cadmium.
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