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Rapid, sensitive, and validated HPLC method for analysis of metronidazole and tinidazole under identical chromatographic conditions with UV detection and liquid-liquid extraction: application in bioequivalence studies

Emami J, Rezazadeh M

Acta Chromatographica

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2013

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Vol. 25, no. 1

111--125

EN

A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of metronidazole and tinidazole in human plasma samples under identical chromatographic conditions. This method involves liquid-liquid extraction using chloroform: isopropylalcohol (95:5). Chromatographic separation was performed using a μ-bondapack C18 (250 mm × 4.6 mm) column. The mobile phase consisted of potassium dihydrogen phosphate solution (0.005 M)/acetonitrile (80/20 v/v). The final pH of the mobile phase was adjusted to 4 ± 0.1 with orthophosphoric acid. The calibration curves were linear over the concentration range 0.1–15 μg/mL for metronidazole and tinidazole with the detection limit of 30 ng/mL. Within- and between-day precision and accuracy did not exceed 9.83% and 10.48%, respectively. Metronidazole and tinidazole were found to be stable in plasma samples with no evidence of degradation during 3 freeze-thaw cycles and 3 months storage in −70 °C. The current validated bio-analytical method was finally applied in bioequivalence studies of two different metronidazole and tinidazole products according to a standard two-way cross-over design with a two-week washout period. No statistically significant difference was observed between the logarithmically transformed AUC0-∞ and Cmax values. Therefore, generic products were considered bioequivalent with those of standards which could be used interchangeably.

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Corrosion inhibition of mild steel in HCl solution by Tinidazole

Reza I., Saleemi A.R., Naveed S.

Polish Journal of Chemical Technology

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2011

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Vol. 13, nr 1

67-71

EN

Tinidazole, a pharmaceutical compound has been investigated with reference to the inhibition of mild steel acidic corrosion in 1 molar HCl by means of weight loss and electrochemical measurements. The outcomes attained at 30°C revealed that the Tinidazole had obtained 90% inhibition efficiency at 400 ppm concentration. These results explain that the inhibition process occurs by means of adsorption. The inhibitor molecules adsorb on the surface of the metal, following Langmuir's adsorption isotherm. Potentiodynamic polarization measurements established that Tinidazole is an inhibitor of a mixed type. An appropriate equivalent electric circuit for modeling and the analysis of impedance data to give a better explanation of the process of corrosion inhibition have been proposed.

3

Sensitive determination of tinidazole using glassy carbon electrode coated with multi-walled carbon nanotube/ionic liquid/chitosan composite film

Yi H., Hu L., Mei P.

Chemia Analityczna

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2009

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Vol. 54, No. 1

19-29

EN

A multi-walled carbon nanotube/l-butyl-3-methylimidazolium tetrafluoroborate/chitosan (MWCNT/IL/CS) composite film - modified glassy carbon electrode (OCR) was fabricated by spin coating. Electrochemical behavior of tinidazole at the composite film electrode was investigated applying voltammetry. A distinct reduction peak located at ca -0.688 V <\ was observed during potential cycling in the range from 0.200 V to -1.00 V. Compared to its voltammetric behavior on bare GCE, the reduction peak potential of tinidazole was shifted positively and the peak current significantly increased. All experimental parameters for electrochemical determination of tinidazole were optimized. The detection limit and linear calibration range were 1.OO.x 10-7mol L-7(S/N = 3) and 3.00x 10-7 to 2.00x 10-5 mol L-1, respectively. In addition, using the proposed film electrode determination of tinidazole in real tablets was carried out with satisfactory results.

PL

Przebadano woltamperometrycznie tynidazol z użyciem elektrody z węgla szklistego zmodyfikowanej warstwą kompozytową składającą się z wielościennych nanorurek węglowych, tetrafluoroboranu l-bulylo-3-metyloimidazolu i chitozanu. Wyraźny pik redukcji pojawił się przy -0,688 V. Porównując ten pik do piku uzyskanego na zwykłej elektrodzie z węgla szklistego stwierdzono, że przesunął się on w kierunku potencjałów dodatnich i znacznie wzrósł. Zoptymalizowano wszystkie parametry do elektrochemicznego oznaczania tynida-zolu. Granica wykrywalności wyniosła 1.00 x 10-7 mol L-1 (S/N = 3), a zakres liniowości krzywej kalibracyjnej określono na 3 x 10-7-2x 10-5 mol L-1. Opracowaną metodę użyto z powodzeniem do oznaczania tynidazolu w tabletkach.