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Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS

Wang Ying, He Yifan, Wang Xianqin, Wen Congcong, Cao Jianbin

Acta Chromatographica

2024

Vol. 36, no. 3

179--185

In this work, a UPLC-MS/MS assay was established for the determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma. ACQUITY UPLC BEH C18 column was employed for chromatographic separation with the mobile phase comprised acetonitrile-10 mmol L1 ammonium acetate aqueous solution (0.05% aqueous ammonia) using gradient elution. Midazolam was used as internal standard (IS). Electrospray ionization (ESI) in positive-ion mode with reaction monitoring (MRM) was used for quantitative analysis. The calibration curves for morphine, codeine, thebaine, papaverine and noscapine demonstrated good linearity (r > 0.995) in the range of 5–500 ng mL1 for morphine and codeine, and 1–100 ng mL1 for thebaine, papaverine and noscapine. The intra-day and inter-day precisions of morphine, codeine, thebaine, papaverine and noscapine were within 15%, the intra-day and inter-day accuracies were 89–114%, the recovery was better than 65%, and the matrix effects were 96–112%. The developed UPLC-MS/MS assay was successfully applied in the pharmacokinetics of papaverine and noscapine.

Construction and performance characteristics of thebaine-selective plastic membrane electrode based on thebaine-Reinecke ion-pair complex

Li Y.-Q., Wen M.-L., Yao Y.

Chemia Analityczna

2002

Vol. 47, No. 2

249-256

The construction and the general performance characteristics of thebaine-selective PVC membrane electrode based on thebaine-reinecke ion-pair complex was described. The electrode exhibited a rapid and Nernstian response (56.7 mV decade') to thebaine hydrochlo-ride concentration ranging from 1 x 10(-6)to I 10(-3)mol dm"1 within pH 1.0-5.0, The lower detection limit was 7.7 x 10(-7)' mol dm(-3). Interfering effects of some foreign substances were reported. The proposed electrode was applied for the direct assay of thebaine hydrochloride content in injections by means of the standard addition method. The results obtained were compared with the determination made by use of high performance liquid chromatography.

Opisano budowę i zbadano charakterystykę elektrody membranowej selektywnej względem tebainy, która zawiera parę je nową: tebaina-sól Reinecke'go w PVC. Elektroda wykazuje nernstowską odpowiedź (56.7 mV dekada(-1) względem chlorowodorku tebainy w zakresie stężeń l x l0(-6) x 10(-3) mol dni(-3) i w zakresie pH 1.0-5.0. Granica wykrywalności wynosi 7.7 x 10(-7) mol dnr'. Wskazano na efekty interferencyjne ze strony niektórych substancji. Elektrodę zasotoswano do bezpośredniego oznaczania chlorowodorku tebainy w zastrzykach, za pomocą metody dodatków. Uzyskane wyniki porównano z wynikami uzyskanymi za pomocą wysokosprawnej chromatografii cieczowej.