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Knitted silk mesh-like scaffold incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with mesenchymal stem cells for repairing Achilles tendon in rabbits

Tang L., Yang Y., Li Y., Yang G., Luo T., Xue Y., Zhang W.

Acta of Bioengineering and Biomechanics

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2018

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Vol. 20, no. 3

77--87

EN

A scaffold knit with natural sericin-free silk fibroin fiber possesses desirable mechanical properties, biocompatibility, ease of fabrication, and slow degradability. However, regenerated silk fibroin degrades faster than natural silk. In this study, natural silk fibroin fiber mesh-like scaffolds were prepared by a weft-knitting method and the pores were filled with sponge-like regenerated silk fibroin-collagen I. The microporous sponge and mesh-like scaffolds were fused to achieve gradient degradation of the scaffolds, and rabbit bone marrow mesenchymal stem cells (BMSCs) were seeded onto the scaffolds to form scaffold–BMSCs composites. The composites were implanted into gap defects made in the rabbit Achilles tendon. Twenty weeks after implantation, histological observation showed that tendon-like tissue had formed, collagen I mRNA was expressed, abundant collagen was generated, and that there was no obvious degradation of silk. The maximum load of the neo-Achilles tendon was 62.14% that of the natural Achilles tendon. These outcomes were superior to those obtained in the group implanted with a scaffold without BMSCs. These findings suggest the feasibility of constructing tissue-engineered tendons using weft-knitted silk scaffolds incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with BMSCs, and show potential of the scaffold–BMSCs composites to repair Achilles tendon defects.

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Charakterystyka zażyciowych i pośmiertnych przemian karbamazepiny oraz wybranych rodzajów alternatywnego materiału biologicznego na potrzeby analiz toksykologiczno-sądowych

Lendor S.

Wiadomości Chemiczne

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2015

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[Z] 69, 7-8

585--603

EN

This article is focused on the data concerning properties of carbamazepine, an antiepileptic drug commonly used in everyday medical practice but also frequently misused leading to many reported cases of intoxication. Due to high prevalence of this drug detailed data concerning its pharmacokinetics and prahmacodynamics can be found as well as metabolic paths that have been proven or have not been confirmed yet. For the purpose of toxicological analyses of samples collected postmortem it is also essential to know the possible transformations of an analyte due to microbial metabolism and postmortem changes of the sample matrix. In forensic cases of unavailable or unusable blood samples when advanced stage of decomposition processes in the found body occurs, alternative matrices are required. Presented review describes three types of such matrices: bones, bone marrow and grease in the context of their postmortem changes and suitability as the source of toxicological information.

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Morphological operations for blood cells extraction from the image of the bone marrow smear

Markiewicz T., Osowski S.

Przegląd Elektrotechniczny

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2008

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R. 84, nr 5

24-26

EN

The paper presents the morphological approach to the extraction of the blood cells on the basis of the image of the bone marrow smear. The basic operation leading to the segmentation of the cells is the watershed algorithm cooperating with the morphological operations like erosion, dilation opening and closing. On the basis of statistical results we have shown that morphological operations represent very efficient tool for the extraction of the blood cells from the medical image.

PL

Praca przedstawia metody przetwarzania morfologicznego obrazu rozmazu szpiku kostnego w zastosowaniu do segmentacji komórek krwiotwórczych. Podstawą ekstrakcji komórek z obrazu jest algorytm działów wodnych współpracujący z podstawowymi operacjami morfologicznymi, takimi jak erozja, dylatacja, otwarcie i zamknięcie. W pracy pokazano, że zastosowanie operacji morfologicznych do segmentacji obrazów medycznych prowadzi do bardzo dobrych wyników wydzielenia poszczególnych komórek i zdecydowanie góruje nad klasycznymi metodami filtracyjnymi.