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Improved adhesion and growth of osteoblast-like MG-63 cells in cultures on titanium modified by gold particles

Parizek M., Base T., Hruby M., Lisa V., Bacakova L.

Engineering of Biomaterials

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2013

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Vol. 16, no. 122-123 spec. iss.

77

EN

Metallic materials are important for load-bearing bone implants. The osteointegration of these implants can be improved by appropriate surface modifications. Therefore, we present here a study of the cell growth on titanium surfaces modified with films created from gold microparticles. These particles in the form of microplates or polyhedral microcrystals were deposited on titanium plates from ethanol solutions, dried and annealed with a hydrogen flame. Some samples were additionally modified by polyethylene imine. The materials engendered from these modifications were used to investigate the adhesion and growth of human osteoblast-like MG-63 cells on these surfaces in the DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surfaces modified by both types of gold microparticles. This trend was the same three and seven days after seeding. The numbers of cells on pure Ti and Ti modified only with gold particles were significantly higher than on samples which were modified with polyethylene imine. The cell spreading areas projected on the materials were significantly larger in cells on the samples with polyethylene imine modification. However, the shape of these cells was mostly rounded or star-like with thin and long protrusions, while on the materials without polyethylene imine, it was mostly polygonal. The cell proliferation activity was estimated from XTT test, based on the activity of mitochondrial enzymes. This test showed that the proliferation activities of osteoblast-like MG-63 cells of the 3rd and 7th days of the experiment were more pronounced on the samples modified only by gold microparticles. Immunofluorescence showed that the focal adhesion plaques containing vinculin and the fibers containing β-actin were most apparent, more numerous and more brightly stained in cells on Ti modified by gold microplates and gold polyhedral microcrystals, especially in comparison with the corresponding samples modified with polyethylene imine (Fig. 1). Thus, it can be concluded that the modification of titanium samples by both types of gold microparticles enhanced the adhesion and growth of MG 63 cells.

2

Biodegradowalne polimery z pamięcią kształtu

Jaros A., Smola A., Kasperczyk J., Dobrzyński P.

Chemik

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2010

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Vol. 64, nr 2

87-96

PL

Praca stanowi krótki przegląd literaturowy dotyczący biodegradowalnych polimerów z pamięcią kształtu (SMP). Scharakteryzowano przebieg i mechanizm efektu pamięci kształtu w tego typu materiałach. Poruszono problem ich biozgodności. Przedstawiono przykłady polimerów SMP będących obiecującym materiałem do zastosowania w formowaniu implantów i narzędzi mogących znaleźć zastosowanie w chirurgii krótkoterminowej.

EN

The article is a brief review of biodegradable shape memory polymers (SMP). It has been described the mechanism of shape memory effect in such materials. The problem of their biocompatibility has been presented. They are enumerated examples of polymers SMP, that are promising material for use in the formation of implants and devices that can be used in short term surgery.

3

The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films

Parizek M., Base T., Londesborough M. G. S., Lisa V., Bacakova L.

Engineering of Biomaterials

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2008

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Vol. 11, no. 81-84

117--119

EN

Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2B10H11 (A), 1,2-(HS)2-1,2-C2B10H10 (B), 9,12-(HS)2-1,2-C2B10H10 (C) and 1,12-(HS)2-1,12- C2B10H10 (D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.

4

The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films

Parizek M., Base T., Londesborough M. G. S., Lisa V., Bacakova L.

Engineering of Biomaterials

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2008

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Vol. 11, no. 75

6--8

EN

Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2 B10H11(A), 1,2-(HS)2-1,2-C2B10H10(B), 9,12-(HS)2-1,2-C2B10H10(C) and 1,12-(HS)2-1,12- C2B10H10(D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.