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1 Environmental Biotechnology

1 2009

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Determination of pathogenic bacteria in wastewater using conventional and PCR techniques

El-Lathy A. M., El-Taweel G. E., El-Sonosy M. W., Samhan F. A., Moussa T. A. A.

Environmental Biotechnology

2009

Vol. 5, No. 2

73-80

The objectives of this investigation were to detect and determine some pathogenic bacteria in raw and treated wastewater and to compare and evaluate different techniques. To achieve this aim, pathogenic bacteria (salmonellae group, total vibrios and Listeria group) were determined in 30 samples collected from inlet and outlet of the treatment plants using PCR, MPN and surface plate techniques. The results of salmonellae group showed that all 30 samples (100%) were positive using PCR technique, 28 samples (about 93%) were positive using direct surface plate technique, and 27 samples (90%) were positive using MPN technique. In case of total vibrios 27 samples (90%) were positive using direct surface plate technique while 28 samples (93%) were positive using MPN technique.When using multiplex PCR technique, detection of three species of Vibrio (V. vulnificus, V. parahaemolyticus, V. cholerae and Classical or El-Tor) were negative. Listeria group was detected in 29 samples (97%) using PCR technique, in 28 samples (93%) using surface plate technique, and in 27 samples (90%) using MPN technique. The removal percentages showed that oxidation pond of El-Sadat City is more efficient in removal of these pathogenic bacteria than the activated sludge system. Statistical analysis (Student t-test) showed that there were significant differences between MPN and surface plate techniques in revealing the three selected pathogenic groups.