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Effects of supersonic treatment on lactoferrin separation from whey waste using reversed micellar system

Inoue H., Yamasaki M., Lakshmi D. S., Kojo T., Yamagishi S. I.

Environment Protection Engineering

2005

Vol. 31, nr 3-4

5-12

A cationic oleic acid reversed micellar system was used to simplify and enhance the purification of bovine lactoferrin (Lfn) from whey. The rate of Lfn extraction by the application of 30 kHz supersonic treatment to the organic solvent phase (P < 0.01) was by 1.5 time higher compared to non-supersonic treatment. An increase in water content and the light scatter were observed in the organic phase when the supersonic treatment was applied. An increase in the efficiency of Lfn extraction by supersonic treatment of an organic phase was attributable to increased water dispersion in the organic solvent phase and an increase in the orientation of the cationic oleic acid towards microparticles of water in organic solvent phase.