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1 International Letters of Chemistry, Physics and Astronomy

1 2013

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Development of an electrochemical method for the measurement of antioxidant capacity of pure compounds and natural substances extracts

Lanez T., Rebiai A.

International Letters of Chemistry, Physics and Astronomy

2013

Vol. 1

46-54

A new method has been developed to measure total antioxidant activity of antioxidants in foods and natural substances without use of standard antioxidants and without use of calibration curves plotting. It is based on measuring the oxidation peak current of superoxide anion radical electrochemically generated by reduction of commercial molecular oxygen in dimethylformamide. The method has been validated using 7 known standard antioxidants and the results have been compared with those obtained by the DPPH and molybdate ion reduction assays. Measured antioxidant capacities were highly correlated with those obtained using DPPH (r2 = 0.549) and molybdate ion reduction assay (r2 = 0.434).