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Preparation of sulfonated polysulfone membrane for enzymes immobilisation

Łukowska E., Wojciechowski C., Chwojnowski A., Dudziński K., Sabalińska S., Ciechanowska A., Czapiewska G.

Biocybernetics and Biomedical Engineering

2012

Vol. 32, no. 2

77-86

The paper presents a method for the preparation of sulfonated polysulfone in order to receive a matrix to immobilisation of biomolecules. Optimum conditions of the synthesis are described. The phase inversion method was used to prepare a sulfonated polysulfone membrane and then urease was immobilized on the surface of the prepared matrix. Comparison of activity of native urease and the immobilized urease is presented.