Wyniki wyszukiwania - BazTech

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Metody analityczne badania preparatów kosmetycznych : wczoraj i dziś

Olejnik Anna, Gornowicz-Porowska Justyna, Gościańska Joanna

Wiadomości Chemiczne

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2022

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[Z] 76, 7-8

607--633

EN

The safety and quality of each cosmetic product is tested before it is introduced to the market. There are highly regulated requirements that producers should comply with. However, as the cosmetic products are highly complexed mixture of different ingredients, detailed characterization of their composition remains still a challenge. Currently, due to the development of modern technologies, a wide range of analytical methods are available. Scientists are increasingly adapting highly sophisticated and advanced techniques to precisely identify and quantify the components of cosmetic products. The aim of this article is to present the analytical methods applied to examine cosmetics taking into account their advantages and limitations. The progress made in recent years in the design of novel instruments leading to their greater efficiency, selectivity and sensitivity was also considered. The techniques that are commonly used in the quality control laboratories of cosmetic companies were presented. Cosmetics analysis is not only limited to the characterization of product itself. Today, in vivo tests are very essential to determine the efficacy of formulations directly on the skin surface. Therefore, in this article modern devices dedicated to theses analyses were described in detail.

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Determination of nilotinib in spiked plasma, urine, and capsules by high-performance liquid chromatography with fluorimetric detection

Yilmaz E. M., Aydoğmuş Z., Aboul-Enein H. Y.

Acta Chromatographica

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2016

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Vol. 28, no. 3

313--331

EN

A precise and sensitive reversed phase high-performance thin-layer chromatography (RP-HPLC) method was developed for the determination of nilotinib (NTB) in spiked plasma, urine, and pharmaceutical capsule formulation. The method was based on derivatization NTB with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in the borax buffer (pH 9). The method employs an isocratic elution using acetonitrile and 10 mM orthophosphoric acid (40:60 v/v) as a mobile phase and an C18 column (4.6 mm × 250 mm, 5 μm, Waters Symmetry), with a fluorescence detector (λex: 447 nm, λem: 530 nm). The method validation was performed with respect to linearity, recovery, accuracy, precision, and stability. The linear ranges were 100–600 ng mL−1 in standard solution, plasma, and urine. Correlation coefficients (r2) were higher than 0.9997 for all of the analytes, indicating good linear relationship. The percentage recovery was 87.89% for plasma, 95.35% for urine, and 96.07% for capsules.

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HPLC analysis of Phyllanthus amarus samples stored in stability chambers under different conditions and study of the effect on quantification of the phytomarkers phyllanthin and hypophyllanthin

Srivastava P., Raut H. N., Puntambekar H. M, Desai A. C

Acta Chromatographica

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2015

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Vol. 27, no. 1

147--156

EN

Storage of medicinal plants may cause deterioration of the active principles with time, thus, reducing the efficacy of plants. Therefore, quantification of the active principle is essential before using the crude drug. Phyllanthus amarus (PA) contains lignans, namely, phyllanthin and hypophyllanthin, which shows anti-hepatotoxic activity. In this paper, we highlight the effect of storage conditions on the quantification of bioactive markers by high-performance liquid chromatography (HPLC) analysis in the crude plant material of PA. HPLC analysis of crude PA samples stored for certain period at long-term study (LS, 30 °C and 65% RH), accelerated study (AS, 40°C and 75% RH), and real-time study (RT) conditions was carried out using the LiChroCART Purospher® STAR RP-18 endcapped (250 × 4.6 mm, 5 μm) column along with a Purospher STAR RP 18e (4.0 × 4.0 mm, 5 μm) guard column using methanol:water (70:30) at a flow rate of 0.7 mL min−1 with ultraviolet (UV) detection at 220 nm. The HPLC study indicated that PA samples kept under LS condition are rich in lignan contents as compared to the samples stored under AS and RT study conditions. Therefore, PA should be used fresh to get maximum concentration of active lignans or it should be stored under LS conditions up to 6 months.

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Use of validated stability-indicating chromatographic methods for quantitative analysis of idrocilamide in a pharmaceutical formulation

Salem M. Y, Din Salama N. N, Abd El-Halim L. M, El-Sayed Abdel Fattah L

Acta Chromatographica

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2010

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Vol. 22, no. 4

569--579

EN

Two sensitive and selective chromatographic methods have been developed and validated for analysis of idrocilamide in the presence of its degradation products. Forced degradation studies were performed using HCl, NaOH, and 3% H2O2. The first method is based on thin-layer chromatographic separation of the intact drug from its degradation products, followed by densitometric measurement. The second method is based on isocratic reversed phase high-performance liquid chromatographic separation of the drug from its degradation products on a C18 column. The HPLC method was used to investigate the kinetics of alkaline degradation of the drug at different temperatures.