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Możliwość rozróżnienia małży i ślimaków słodkowodnych na podstawie koncentracji metali ciężkich w tkankach miękkich i muszlach

Piotrowski S.

Przegląd Geologiczny

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2017

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Vol. 65, nr 6

400--404

EN

The study involved 17 species of freshwater molluscs. The concentration of heavy metals (Cu, Zn, Pb, Co, Cd andHg) in soft tissues was determinedfor 14 species, while the concentration of heavy metals in shells was determinedfor 16 species. Totally, 110 samples of soft tissue and 119 samples of shells were analysed. The statistical analysis of the qualitative data concerning concentration of metals in shells indicated a clear distinction between snails and bivalves. The only exception is Unio crassus, which was assigned to a three-point cluster together with snails. With respect to snails, this may be because the analysis was performed only on one sample. The results of this study can be significant both for palaeontological research and palaeoenvironmental research. If we have a few shell fragments available, it can be generally concluded, based on geochemical analysis of heavy metals in shells, which shell fragments belong to bivalves and which to snails. It is also possible to assess geochemical conditions of ancient ecosystems. Obviously, the given results are preliminary and they suggest necessity of further research.

2

Application of High-Resolution 3D Scanning in Medical Volumetry

Chromy A.

International Journal of Electronics and Telecommunications

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2016

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Vol. 62, No. 1

23--31

EN

This paper deals with application of 3D scanning technology in medicine. Important properties of 3D scanners are discussed with emphasize on medical applications. Construction of medical 3D scanner according to these specifications is described and practical application of its use in medical volumetry is presented. Besides volumetry, such 3D scanner is usable for many other purposes, like monitoring of recovery process, ergonomic splint manufacturing or inflammation detection. 3D scanning introduces novel volumetric method, which is compared with standard methods. The new method is more accurate compared to present ones. Principles of this method are discussed in paper and its accuracy is evaluated and experimentally verified.

3

Zastosowanie mięczaków słodkowodnych jako wskaźników zanieczyszczenia środowiska wodnego metalami ciężkimi (Cu, Zn, Pb, Co, Cd, Hg)

Piotrowski S.

Inżynieria i Ochrona Środowiska

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2015

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T. 18, nr 4

513--524

PL

Badaniami objęto 17 gatunków mięczaków słodkowodnych, z czego dla 14 gatunków oznaczono koncentracje metali ciężkich (Cu, Zn, Pb, Co, Cd i Hg) w tkankach miękkich, a dla 16 gatunków oznaczono stężenia tych pierwiastków chemicznych w muszlach. Łącznie dysponowano wynikami analiz koncentracji metali w 110 próbkach tkanek miękkich i w 119 próbkach muszli. Gatunkami, których tkanki miękkie i muszle najlepiej reagują na zmiany koncentracji metali ciężkich w wodzie i osadach dennych, okazały się: Anodonta anatina, Dreissena polymorpha, Lymnaea stagnalis, Pseudoanodonta complanata i Viviparus contectus. Drugą grupę, odzwierciedlającą w mniejszym stopniu poziomy metali w środowisku, stanowią cztery gatunki: Viviparus viviparus, Sphaerium solidum, Unio pictorum oraz Unio tumidus. Gatunkami najlepiej odzwierciedlającymi poziomy metali w środowisku w poszczególnych układach środowiskowych są: (1) tkanki - woda: Viviparus contectus, Anodonta anatina oraz Viviparus viviparus, (2) tkanki - osady: Anodonta anatina, Pseudoanodonta complanata i Viviparus contectus, (3) muszle - woda: Dreissena polymorpha, Unio pictorum i Lymnaea stagnalis, (4) muszle - osady: Viviparus viviparus, Dreissena polymorpha i Sphaerium solidum. W wodzie powierzchniowej wszystkie sześć badanych metali możemy kontrolować poprzez analizę muszli Dreissena polymorpha, a w osadach dennych Cu, Zn, Pb, Cd i Hg poprzez analizę tkanek oraz Co poprzez analizę muszli Anodonta anatina. Te dwa gatunki wydają się podstawowe do dalszych prac. Dwa gatunki uważa się za gatunki uzupełniające w dalszych pracach. Są to: Lymnaea stagnalis (w wodzie możemy kontrolować Cu, Zn, Pb, Cd poprzez analizę muszli, a w osadach Cu, Pb, Cd poprzez analizę muszli oraz Zn poprzez analizę tkanek), Viviparus contectus (w wodzie możemy kontrolować Cu, Co, Cd poprzez analizę tkanek i Pb poprzez analizę muszli, a w osadach Cu, Zn, Co i Cd poprzez analizę tkanek). W toku dalszych prac należy zrezygnować z gatunków, które od 2001 r. są na liście gatunków chronionych w Polsce. Są to: Sphaerium rivicola, Sphaerium solidum, Unio crassus, Pseudoanodonta complanata oraz Anodonta cygnea.

EN

The research was applied to 17 species of freshwater molluscs, for 14 species the concentration of heavy metals (Cu, Zn, Pb, Co, Cd and Hg) was marked in soft tissues, and for 16 species the concentration of these metals was marked in shells. This study is based on the analysis of 110 samples of soft tissues and 119 samples of shells. The species that best react to the changes in concentration of the heavy metals in water and sediments are: Anodonta anatina, Dreissena polymorpha, Lymnaea stagnalis, Pseudoanodonta complanata and Viviparus contectus. The second group, which is less related with the environment, are four species: Viviparus viviparus, Sphaerium solidum, Unio pictorum and Unio tumidus. The species most related with the environment in individual arrangements are: (1) tissues - water: Viviparus contectus, Anodonta anatina and Viviparus viviparus, (2) tissues - sediments: Anodonta anatina, Pseudoanodonta complanata and Viviparus contectus, (3) shells - water: Dreissena polymorpha, Unio pictorum and Lymnaea stagnalis, (4) shells - sediments: Viviparus viviparus, Dreissena polymorpha i Sphaerium solidum. In the surface water we can control all six analyzed metals through the shell analysis of Dreissena polymorpha, and in the bottom sediments we can control the concentration of Cu, Zn, Pb, Cd i Hg by soft tissues analysis and Co by shell analysis of Anodonta anatina. These two species are crucial for the future research. Two other species are considered as supplementary. Those are: Lymnaea stagnalis (in water we can control the concentration of Cu, Zn, Pb, Cd by shell analysis, and in sediments we can control Cu, Pb, Cd by shell analysis and Zn by soft tissue analysis), Viviparus contectus (in water we can control the concentration of Cu, Co, Cd by tissues analysis and Pb by shell analysis, and in sediments we can control Cu, Zn, Co i Cd by tissues analysis). In the future research one should resig from using five species that are under protection in Poland since 2001. Those are: Sphaerium rivicola, Sphaerium solidum, Unio crassus, Pseudoanodonta complanata and Anodonta cygnea.

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Mechanobiology of soft tissues: FT-Raman spectroscopic studies

Gąsior-Głogowska M., Komorowska M., Hanuza J., Mączka M., Będziński R., Kobielarz M.

Challenges of Modern Technology

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2011

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Vol. 2, no. 3

8--11

EN

FT-Raman spectroscopy was used to investigate microstructural changes in the secondary protein structure of soft tissues subjected to increasing levels of macroscopic strain. Main protein bands at 938 cm-1 assigned as v(Cα–C), 1668 cm-1 — amide I and 1268 cm-1 — amide III are sensitive to applied strain and undergo wavenumber shifting. Other main vibrational modes at 1004 cm-1 assigned to the phenyl ring breathing mode and 2940 cm-1 (n(CH3,CH2)) remain unaltered. Spectroscopic results were compared with the mechanical relations obtained from the standard protocol of uniaxial tensile tests carried out in a testing machine. A clear correlation between Raman band shifting and the level of mechanical stress was established. Initially the load is transferred through elastin and then gradually also by collagen. It was proved that transferring loads by soft tissues involves changes in structural protein conformation. This process was described in detail for a tendon. It was also confirmed that mechanical properties of soft tissues depend on collagen and elastin fiers orientation.

5

Przydatność Anodonta anatina (L.) jako organizmu wskaźnikowego odzwierciedlającego poziomy metali ciężkich (Cu, Zn, Pb, Co, Cd, Hg) w wodzie i osadach dennych

Piotrowski S.

Inżynieria i Ochrona Środowiska

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2010

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T. 13, nr 4

259-277

PL

Celem niniejszego artykułu było stwierdzenie, w jakim stopniu tkanki miękkie i muszle Anodonta anatina (L.) (szczeżuja pospolita) odzwierciedlają zmiany poziomów Cu, Zn, Pb, Co, Cd i Hg w wodzie powierzchniowej i osadach dennych (frakcja poniżej 0,20 mm) ujścia Odry. Materiał do badań laboratoryjnych został pobrany w latach 1999 i 2000 z obszaru ujścia Odry (19 stanowisk) oraz z jeziora Miedwie (jedno stanowisko). Na każdym stanowisku oznaczano koncentracje metali w wodzie powierzchniowej, osadach dennych (frakcja poniżej 0,20 mm) oraz w tkankach miękkich (całe ciało) i muszlach Anodonta anatina. Analizowano 20 próbek tkanek miękkich i 12 próbek muszli Anodonta anatina oraz po 20 próbek wody powierzchniowej i osadów dennych. Na każdym stanowisku analizowano próbki Anodonta anatina obejmujące co najmniej kilkanaście okazów o różnych rozmiarach (tzw. próbki uśrednione). Oznaczenia Cu, Zn, Pb, Co, Cd dokonano techniką ICP-AES, a Hg oznaczono techniką CV-AES. Przy zastosowaniu Anodonta anatina, jako organizmu wskaźnikowego, rejestruje się zmiany poziomów stężeń metali ciężkich w wodzie powierzchniowej na podstawie analizy tkanek miękkich i muszli (Cu i Zn), analizy muszli (Pb i Hg) oraz analizy tkanek (Hg). Z sześciu badanych metali nie kontroluje się tylko zmian Co. Z kolei zmiany koncentracji metali w osadach można kontrolować, wykorzystując tkanki miękkie i muszle (Zn i Hg), tylko tkanki (Cu, Pb i Cd) i tylko muszle (Co).

EN

The aim of the present article was the determination to what extent soft tissues and shells of the Anodonta anatina (Anodonta cygnea piscinalis NILSSON 1823; Anodonta piscinalis NILSSON 1823) reflect the changes Cu, Zn, Pb, Co, Cd and Hg in superficial water and bottom sediments (fraction below 0.20 mm) of the Odra River estuary. The material for laboratory investigations was taken in years 1999 and 2000 of the Odra River estuary (19 research points) and from Lake Miedwie (one research point). The concentrations of metals were marked at every research point in superficial water, bottom sediments and in soft tissues (whole body) and shells of the Anodonta anatina. 20 samples of soft tissues, 12 samples of the shells of the Anodonta anatina and 20 the samples of superficial water and bottom sediments were analysed. Samples of the Anodonta anatina (averaged samples) were analysed at every research point consisting more than ten specimens of various sizes. Quantitative determination Cu, Zn, Pb, Co, Cd was executed with the technique ICP-AES and Hg was marked with the technique CV-AES. The changes of the levels of the concentrations of heavy metals in superficial water were correlated with the results of the analysis of soft tissues and shells (Cu and Zn), the analysis of shells (Pb and Hg) and the analysis of soft tissues (Hg). Only changes of the Co did not show appropriate correlation. The soft tissues and shells (Zn and Hg), only soft tissues (Cu, Pb and Co) and only shells (Co) can reflect the changes of concentration of metals in bottom sediments.

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Temperature Fields Induced by Low Power Focused Ultrasound in Soft Tissues During Gene Therapy. Numerical Predictions and Experimental Results

Gambin B., Kujawska T., Kruglenko E., Mizera A., Nowicki A.

Archives of Acoustics

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2009

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Vol. 34, No. 4

445-459

EN

The aim of this work is twofold. Firstly, to verify a theoretical model which is capable of predicting temperature fields appearing in soft tissues during their ultrasound treatment. Secondly, to analyze some aspects of the dynamics of Heat Shock Response induced by the heating process in the context of therapeutic treatment. The theoretical investigations and quantitive analysis of temperature increments at any field point versus time of heating process, depending on the heat source power, spatial distribution and duration as well as on the tissue thermal properties, has been carried out by Finite Element Method (FEM). The validation of the numerical model has been performed by comparison of the calculation results with the experimental data obtained by measuring in vitro of the 3D temperature increments induced in samples of the turkey and veal liver by the circular focused transducer with the diameter of 15 mm, focal length of 25 mm and resonance frequency of 2 MHz. Various ultrasonic regimes were considered. They were controlled by adjusting ultrasound power and exposure time. The heat shock proteins (HSP) and misfolded proteins (MFP) levels during the proposed cyclic sonification are presented.

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The development and manufacture of polymeric endoprosthetic meshes for the surgery of soft tissues

Zhukovsky V., Rovinskaya L., Vinokurova T., Zhukovskay I.

Autex Research Journal

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2002

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Vol. 2, no. 4

204--209

EN

'Esfil' endoprosthetic meshes made of polypropylene monofilaments, 'Eslan' made of lavsan multifilaments and 'Ftorex' made of lavsan multifilaments with waterproofing fluoropolymer coating have been developed and manufactured for the plasty of soft supporting tissues after tumour resection and herniotomies, by damage to the abdominal wall and the diaphragm and in other surgical procedures. 'Esfil' polypropylene endoprostheses are biologically inert and resist the action of tissue fluids. The hydrophoby and solidity of the threads prevent the meshes from becoming infected. Prompt spreading of the fibroblasts along the filaments promotes the reparation of soft tissues. The 'Eslan' meshes are substantially softer and can be used when so-called 'gentle' implants are required. However, the capillarity of lavsan filaments may cause wound infections. The 'Ftorex' prostheses do not suffer from this disadvantage, due to impregnation of the pores between multifilaments with fluoropolymer. At the same time, the manipulation properties of the prostheses remain stable and their biocompatibility and bioresistance increase. Physico-mechanical, medical, biological and clinical tests allowed us to determine the optimal knitting structures of the endoprostheses we developed.