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Inhibitory Effect of Sodium Benzoate as Preservative Material in the Biogas Production in a Batch Anaerobic Digestion Process

Sumantri Indro, Buchori Luqman, Anggoro Didi Dwi

Journal of Ecological Engineering

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2020

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Vol. 21, nr 8

120--128

EN

Sodium benzoate has been used a food preservative worldwide. The effect of sodium benzoate as a preservative in the wastewater treatment was examined from the biogas formation viewpoint. The research was conducted in batch mode reactor systems employing various ratios of activated sludge and solution of sodium benzoate volume. The MLSS of activated sludge used was 12 g/L, while the volume ratios of activated sludge and sodium benzoate ranged from 0 to 100%. The concentrations of sodium benzoate used were 50, 100, and 200 mg/L. The biogas samples were measured every two days for 60 days. The results showed that the volume ratio of activated sludge and sodium benzoate of 60% and 40% was a turning point where the existence of sodium benzoate influenced the formation of biogas. There were significant reductions of biogas formation from 200.6 mL to 66.6 mL, 159.8 mL to 66.0 mL and 130.2 mL to 54.0 mL for the initial SB concentrations of 50, 100, and 200 mg/L. The kinetic parameters of the Modified Gompertz equation exhibited the greatest degree of confidence equal to 95%.

2

Development and validation of a new isocratic RP-HPLC method for simultaneous determination of sodium metabisulfite and sodium benzoate in pharmaceutical formulation

Ivković B., Brborić J., Dobričić V., Čudina O.

Acta Chromatographica

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2019

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Vol. 31, no. 2

133--137

EN

A simple and convenient reversed-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous separation, identification, and determination of sodium metabisulfite and sodium benzoate in pharmaceutical formulation has been developed and validated. Chromatographic separation was achieved on RP column Zorbax Extend C-18 (150 × 4.6 mm i.d., 3.5 μm particles), and mixture of 0.1% phosphoric acid and acetonitrile in the ratio 62:38 (v/v) was used as a mobile phase. The flow rate was set at 1.0 mL/min with detection wavelength of 275 nm. The method was successfully validated according to International Conference on Harmonization (ICH) guidelines acceptance criteria. The method is selective, as no interferences were observed at retention times corresponding to these analytes. Results of regression analyses (r) and statistical insignificance of calibration curve intercepts (p) proved linearity of the method in defined concentration ranges for sodium metabisulfite and sodium benzoate (0.05–0.15 mg/mL). Relative standard deviations calculated for both analytes in precision testing were below the limits defined for active pharmaceutical ingredients (analysis repeatability: <2%; intermediate precision: <3%). Recovery values were between 98.16% and 101.94%. According to results of robustness testing, chromatographic parameters are not significantly influenced by small variation of acetonitrile content in mobile phase, column temperature, and flow rate. Finally, the method was applied for quantitative determination of investigated preservatives in real sample analysis.

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Development of reverse-phase high-performance liquid chromatography method for simultaneous determination of sodium benzoate and potassium sorbate in beverages

Petanovska-Ilievska B., Velkoska-Markovska L., Jankulovska M.

Acta Chromatographica

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2017

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Vol. 29, no. 3

345--358

EN

Reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of sodium benzoate and potassium sorbate in beverages was developed using high speed column. The simple and rapid reverse-phase method for quantitative determination of both preservatives was established on LiChroCART® Purospher STAR RP-18e (30 mm × 4 mm; 3 μm) column, mobile phase consisted of acetonitrile-phosphate buffer (pH = 3.5) in volume ratio of 8:92 (v/v), flow rate of 1 mL min−1, ultraviolet (UV) detection at 195 nm for sodium benzoate and 260 nm for potassium sorbate, and constant column temperature at 25 °C. Linearity, precision, accuracy, limit of quantification (LOQ), and limit of detection (LOD) were tested for method validation. Linearity range for sodium benzoate was 6.04–200.27 mg L−1 (R2 = 0.999) while, for potassium benzoate (R2 = 0.999), 12.19–406.36 mg L−1. The RSD values ≤1.03% demonstrate excellent intra-day precision. LOD for sodium benzoate and potassium sorbate was 0.004 and 0.003 mg L−1, while LOQ was 0.012 and 0.009 mg L−1, respectively. This method was applied for quantitative determination of investigated preservatives in beverages which were taken from Macedonian markets.

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Inhibitory effects of sodium benzoate on germination and growth seedling of radish Raphanus sativus L.

Święciło A.

Ecological Chemistry and Engineering. A

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2008

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Vol. 15, nr 3

245-251

EN

Derivatives of benzoic acid are produced on industrial scale and are used as preservatives in food, pharmaceutical and cosmetic industry. They also occur naturally in same berries and are intermediates of metabolic paths. In over-physiological doses they have a destructive effect on human body and other live organisms. The aim of Ibis study was to determine the influence of sodium benzoate on germination process of radish seeds Raphanus sativus L. The results that were obtained reveal that sodium benzoate in the concentration lower than 0.03 mol. dm-3 decreases the number of germinated seeds of radish. Seedlings obtained from these seeds were shorter than the seedlings in control and their roots were also reduced. No other pathomorphological features were observed. Sodium benzoate used in the concentration equal to or higher than 0.03 mol. dm-3 inhibits the germination process of radish seeds but it does not decrease the viability of germs of these seeds and does not change their catalase activity. The process of inhibiting germination can be reversed by removing Ibis substance from the environment.

PL

Pochodne kwasu benzoesowego produkowane na skalę przemysłową są wykorzystywane jako substancje konserwujące głównie w przemyśle spożywczym, farmaceutycznym i kosmetycznym. Występują one także w stanie naturalnym w niektórych owocach jagodowych oraz są intermediatami szlaków metabolicznych. W nie fizjologicznych dawkach działają szkodliwie na organizm człowieka i inne organizmy żywe. Celem niniejszej pracy było badanie wpływu benzoesanu sodu na proces kiełkowania nasion i wydłużania korzeni u rzodkiewki Raphanus sativus L. Uzyskane wyniki wskazują, że benzoesan sodu zastosowany w stężeniach mniejszych niż 0,03 mol. dm-3 obniża liczbę wykiełkowanych nasion rzodkiewki. Siewki uzyskane z tych nasion były mniejsze niż siewki kontrolne i charakteryzowały się skróceniem korzenia. Nie stwierdzono u nich innych cech patomorfologicznych. Benzoesan sodu zastosowany w stężeniu równym lub większym niż 0,03 mol. dm-3 hamuje proces kiełkowania nasion rzodkiewki, ale nie obniża żywotności zarodków tych nasion i nie zmienia ich aktywności katalazowej. Proces hamowania kiełkowania można odwrócić poprzez usunięcie tej substancji ze środowiska.

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Oddziaływanie benzoesanu sodu na wybrane procesy fizjologiczne w kiełkujących nasionach rzodkiewki Raphanus sativus L.

Święciło A.

Proceedings of ECOpole

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2007

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Vol. 1, No. 1/2

253--257

PL

Zbadano wpływ benzoesanu sodu na proces kiełkowania nasion rzodkiewki Raphanus sativus L. Uzyskane wyniki sugerują, że benzoesan sodu w stężeniach mniejszych niż 0,03 mol • dm-3 obniżał energię, a w mniejszym stopniu zdolność kiełkowania nasion rzodkiewki. Nie modyfikował jednak dynamiki wzrostu siewek i nie indukował cech patomorfologicznych. Natomiast zastosowany w stężeniach większych niż 0.03 mol • dm-3 całkowicie hamował proces kiełkowania nasion. Zarodki tych nasion były żywe, ale charakteryzowały się małą aktywnością katalazy w porównaniu do nasion skiełkowanych.

EN

Derivatives of benzoate acid are produced at industrial level and are used as preservatives in food, pharmaceutical and cosmetic industries. They also occur naturally in some berries and are intermediates of metabolic trails. In over-physiological doses they have a destructive effect on human body and other living organisms. The aim of this study was determine the influence of sodium benzoate on germination processes of radish seeds Raphanus sativus L. The results that were obtained reveal that sodium benzoate in concentration lower than 0.03 mol • dm-3 decreases energy, and to a smaller extent germination ability of radish seeds. However, sodium benzoate does not modify growth dynamics of seedlings, nor does it induce pathomorphological features. In concentration higher than 0.03 mol • dm-3 sodium benzoate totally inhibits seed germination process. Despite the lack of signs of seed germination in the investigated samples, no decrease in germ vitality in the seeds was observed. The results suggest that biological tests based on seed germination process can be used to determine potential toxicity of preservatives.

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Effect of sodium benzoate (E211) and sodium citrate (E331) on chosen biological characters of the fruit fly (Drosophila melanogaster)

Mitrus J., Koczkodaj D.

Chemia i Inżynieria Ekologiczna

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2007

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Vol. 14, nr 8

847-853

EN

Searching of new methods for enhance of food quality, and extension of its shelf-life, in food industry various additives are used. Sodium benzoate is a widely used as food preservative for extending shelf-life of sauces, ketchups and soft drinks. Sodium citrate is applied as a acid regulator, stabilizer or an antioxidant. Usually it is added to jams, jellies, cheeses, and sausages. Both additives are often used simultaneously. The fruit fly (Drosophila melanogaster) can be used for studies confirming safety of additives for food. In experiments the wild tribe of Drosophila melanogaster was used. The influence of two doses of sodium benzoate (E211) and sodium citrate (E331) added simultaneously to feed of fruit fly on its biology were investigated. Two concentrations of the feed additives were tested - lower: 0.8 mg o kg-1 of (E211), and 2.0 g kg-1 of E331, and higher: 1.6 mg kg-' of E211 and 4.0 g kg-' of E33. Simultaneously added to feed sodium benzoate (E211) and sodium citrate E331 (at doses: 0.8 mg o kg-' and 2.0 go kg-', and 1.6 mg . kg-' end 4.0 go kg -', respectively) had no effect on fertility in most of studied generations of wild tribe fruit fly. Used doses of E211 and E331 had no influence on population of most generations of fruit fly too, as well as on body weight of males and females. Only in two generations number of females and males was higher than in control population. Studied additives have no effect on inheritance of wild tribe of Drosophila melanogaster.

PL

W poszukiwaniu nowych metod technologicznych, pozwalających na podniesienie jakości produktu oraz przedłużenie jego trwałości, w przemyśle spożywczym stosowane są dodatki do żywności. Warunkiem stosowania danego dodatku do żywności jest stwierdzenie braku jego wpływu na zdrowie konsumentów. Wydaje się, że muszka owocowa (Drosophila melanogaster) może być dobrym obiektem dla prowadzenia tego typu badań. Celem przeprowadzonych dwuletnich eksperymentów była ocena wpływu benzoesanu sodu (E211) i cytrynianu jednosodowego (E331) na biologię dzikiego szczepu muszki owocowej. Badania prowadzono przez dziesięć pokoleń Drosophila. Benzoesan sodu jest powszechnie używanym konserwantem dodawanym m.in. do sosów, keczupów i napojów. Cytrynian jednosodowy jest stosowany jako regulator kwasowości, stabilizator oraz przeciwutleniacz. Najczęściej dodaje się go do dżemów, marmolad, galaretek, serów topionych, kiełbas i konserw mięsnych. Podczas przedstawionych badań zastosowano dwa stężenia powyższych dodatków do żywności: 0,8 mg o kg-' i 1,6 mg o kg- 1 benzoesanu sodu (E211) oraz 2,0 go kg-' i 4,0 go kg-' cytrynianu jednosodowego (E331). Z analizy otrzymanych danych wynika, iż przy dawkach minimalnych zalecanych (0,8 mg - kg-' E211 i 2,0 go kg-' E331) w żywności substancje te nie wpływały znacząco na plenność samic, masę ciała i strukturę płci Drosophila melnogaster.