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EN
This research aimed to investigate the optimum conditions of slow sand filter (SSF) media modification by using ground Anadara granosa shell waste and the effect of the ripening period on the total coliform (TC) removal efficiency. The response surface methodology with the central composite design was conducted with three factors, namely, seeding time (2–3 weeks), running time (0–20 days), type of SSF media (i.e., river sand, A. granosa shell, and their combination), as independent variables. The results showed that the ripening period factor interacted insignificantly by improving the TC removal efficiency due to short ripening time (p > 0.05). The optimum conditions of the SSF to achieve maximum TC removal efficiency (99.70 ± 21.50%) were as follows: combination media of river sand and ground A. granosa shell waste, 2.8 weeks (20 days) of ripening period, and 20 days of operation. In conclusion, the optimum operating parameters of the slow sand filter revealed that the combination of river sand and A. granosa shell as well as prolonged ripening and running times could increase the removal efficiency of TC. Hence, the A. granosa shell has good application potential as filter media to remove TC from the municipal wastewater.
EN
This research aimed to discover the macroscopic, microscopic, and physiological characteristics and the genus of heterotrophic bacteria found in the schmutzdecke or biofilm layer in slow sand filters. The isolation of heterotrophic bacteria in the schmutzdecke applied the pour plate and quadrant streak method, while the characterization used macroscopic, microscopic, and physiological tests. Samples were taken from the schmutzdecke on top of the filter layer, and they were diluted 3, 4, and 5 times, then grown in Nutrient Agar media in order to isolate heterotrophic bacteria. The results of the research were analyzed using the identification manual books titled Bergey’s Manual of Determinative Bacteriology, 9th edition and Manual for The Identification of Medical Bacteria, 3rd edition. These manuals show the names of the genus of bacteria in the schmutzdecke layer. On the basis of the identification results from macroscopic, microscopic, and physiological tests, there were 4 dominant genera out of 18 living isolates obtained from the schmutzdecke layer, namely Kurthia gibsonii, Bacillus badius, Bacillus firmus, and Bacillus lentus. The similarity percentage of these 4 isolates was 83%, 81%, 85%, and 77% respectively.
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