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Spektroskopia pojedynczych fluoroforów

Molski A.

Wiadomości Chemiczne

2006

[Z] 60, 7-8

449-465

This article is an introduction to single-fluorophore spectroscopy (SFS). The basic phenomenon seen in SFS is the fluctuations of fluorescence intensity of single nano-objects (dyes, biopolymers, nano-crystals, aggregates). These fluctuations are called fluorescence blinking and carry information on the dynamics of a fluorophore and its surroundings. In the simplest case the fluorescence trajectory jumps between two levels, a bright one (on) and a dark one (off). In this article on-off fluorescence blinking associated with triplet states of organic dyes, excitation of quantum dots, and enzymatic reactions at room temperatures are reviewed. The cited literature indicates current problems and may serve as an introduction to modelling and simulations of SFS.