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TLC–Densitometry analysis of indole compounds in mycelial culture of Imleria badia and Agaricus bisporus enriched with precursors — serine or anthranilic acid

Opoka W., Kała K., Krężałek R., Sułkowska-Ziaja K., Maślanka A., Muszyńska B.

Acta Chromatographica

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2018

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Vol. 30, no. 4

236--242

EN

Agaricus bisporus and Imleria in vitro cultures were cultivated on modified Oddoux medium, and Oddoux medium was enriched with serine or anthranilic acid. Serine or anthranilic acid was used at the concentrations of 0.1, 0.25, 0.5, and 0.75 g/L of medium. Determination of indole compounds in the obtained biomass was carried out using thin-layer chromatography (TLC) with densitometric detection. In every analyzed sample, presence of serine or anthranilic acid was studied. Comparison of the results obtained for the treatment and control samples allowed us to determine the optimum concentration of serine or anthranilic acid in the medium in order to obtain biomass with increased content of indole compounds. A. bisporus with addition of anthranilic acid or serine to the medium at the concentration of 0.5 g/L was the most beneficial. In the case of Imleria badia, anthranilic acid at the concentration of 0.5 g/L was the most optimal. This is the first report demonstrating the content of indole derivatives in biomass affected by their precursors (serine or anthranilic acid). The study indicates that modification of the medium can provide satisfactory results, and it is worth to search for its new, improved compositions.

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Analysis of the enantiomers of serine by reversed-phase high-performance liquid chromatography with OPA-Boc as pre-column derivatization agent. Critical evaluation of the analysis of d-serine in rat brain

Oláh E, Berky R, Fekete J

Acta Chromatographica

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2011

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Vol. 23, no. 1

59--68

EN

The enantiomers of serine have been separated after pre-column derivatization with ortho-phthaldialdehyde (OPA) and N-tert-butyloxycarbonyl-L-cysteine (Boc). Analysis of the enantiomers of amino acids after pre-column derivatization with OPA-Boc has been reported in the literature but no critical evaluation has been carried out. In this study, decomposition of the diastereomers, effect of the sample pH, and amount of derivatization agent were studied; the half life of the decomposition was also determined. The method was used for analysis of D and L-serine from three different areas of rat brain (the striatum, prefrontal cortex, and cerebellum).

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Synthesis and characterization of alumina nanopowders by combustion of nitrate-amino acid gels

Edrissi M., Norouzbeigi R.

Materials Science Poland

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2007

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Vol. 25, No. 4

1029--1040

EN

Nanocrystalline alumina powders were synthesized by the combustion method using serine and asparagine as fuels. A screening design was conducted to determine how key process factors influence preparation of nanocrystalline powders. The screening design was utilized to rank effective factors on crystalline size of alumina powders. The product was characterized by XRD, BET, and SEM. Nanocrystalline ?-alumina powders with crystal sizes between 3.95 nm and 6.71 nm and ?-alumina powders with crystallite sizes between 22.73 nm and 33.92 nm have been obtained by the combustion synthesis. The specific surface areas of samples ranged between 22 m2/g and 75 m2/g. Particle size distributions were determined by LLS and the average particle sizes of ?-alumina powders after sonication were 37.42 nm and 79.32 nm. Results of statistical analysis illustrate that the fuel to oxidizer ratio is the most effective factor to decrease the average crystal size.