For the first time the 31P solid-state NMR CP/MAS technique was applied to determine the amount of C-P forms in the total phosphorus in sea anemones. Signals of phosphonates (ca 20 ppm) and phosphates (ca 0 ppm) are well resolved and the phosphonate/ phosphate ratios were easily calculated. Sea anemones Anemonia sulcata from the Adriatic Sea contain 41-54% of phosphorus as phosphonates (P-C). 31PNMRanalysis of lipids was also performed.
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