Wyniki wyszukiwania - BazTech

1

Aktywność przeciwgrzybicza preparatu śliny zawierającego nanocząstki złota

Mystkowska J., Niemirowicz K., Tokajuk G., Łysik D., Lewkowicz E., Dąbrowski J. R., Bucki R.

Engineering of Biomaterials

|

2016

|

Vol. 19, no. 137

20--29

PL

Ślina naturalna jest jednym z najważniejszych płynów ustrojowych i pełni wiele istotnych funkcji w jamie ustnej. Jednakże, w pewnych sytuacjach, dochodzi do procesu zaburzenia jej wydzielania, co skutkuje pojawieniem się objawów hyposaliwacji (redukcji wydzielania śliny) lub kserostomii (suchość jamy ustnej). W takich przypadkach stosuje się substytuty śliny, których zadaniem jest pełnienie funkcji śliny naturalnej. W niniejszej pracy przygotowano i przetestowano preparaty śliny, których skład chemiczny oparty jest o mucynę pochodzenia zwierzęcego i gumę ksantanową, rozpuszczonych w buforowanym roztworze soli. Przygotowane preparaty śliny wzbogacono o nanocząstki złota, uzyskując roztwory o stężeniu 200 μg/ml. Następnie, przeprowadzono badania ich aktywności przeciwgrzybicznej (w stosunku do szczepu Candida albicans). Do badań biologicznych wykorzystano próbki materiału implantacyjnego powszechnie stosowanego w protetyce stomatologicznej (stop Co-Cr-Mo, Remanium 380+), który przetrzymywano w roztworze kontrolnym i preparatach śliny (z/bez dodatku nanocząstek złota) w czasie 72 h w temperaturze 37°C. Po inkubacji dokonano obserwacji mikroskopowych powierzchni próbek badanego stopu z wykorzystaniem konfokalnego laserowego mikroskopu skaningowego (CLSM). Uzyskane wyniki badań wykazują różnice pomiędzy testowanymi roztworami w zakresie obecności drobnoustrojów Candida albicans. Zaobserwowano, że obecność nanocząstek złota w preparacie śliny istotnie redukuje wzrost szczepu Candida na powierzchni testowanego stopu dentystycznego.

EN

Natural saliva is one of the most important biological fluids and it fulfils many important functions in oral cavity. However, in some cases, disorders of salivary gland functions may cause hyposalivation (reduction of saliva secretion) or xerostomia (dryness of the oral cavity). Thus, so-called saliva substitute preparations can be used to support the functions of saliva. In our work, saliva substitute was prepared on the basis of porcine gastric mucin and xanthan gum, both dissolved in saline buffer. Additionally, gold nanoparticles were added to tested solution with concentration of 200 μg/ml and biological characterisation was evaluated. Analysis of the candidacidal activity (against Candida albicans) of prepared saliva substitute with addition of gold nanoparticles was performed. Tests were carried out after keeping samples of dental material (Co-Cr-Mo alloy, Remanium 380) in control sample and saliva preparations (with and without gold nanoparticles) during 72 hours at 37°C. After incubation, tested surfaces were observed using confocal laser scanning microscopy (CLSM). The obtained results of microscopic observations showed differences between the tested preparations in the scope of presence of Candida albicans. It was observed that gold nanoparticles significantly reduced the growth of Candida albicans species at tested dental material.

2

Influence of self-made saliva substitutes on tribological characteristics of human enamel

Andrysewicz E., Mystkowska J., Dąbrowski J. R, Olchowik R.

Acta of Bioengineering and Biomechanics

|

2014

|

Vol. 16, no. 2

67--74

EN

This paper describes the results of tests on the influence of human saliva and its substitutes on tribological characteristics of friction pairs. Each pair consists of enamel and one of the following materials: ceramics, the Meridian B2 dental composite, the GK dental amalgam, and Ti-6Al-4V titanium alloy. The saliva substitutes used were prepared using pyrophosphates, xanthan gum, and mucins dissolved in a saline buffer. The results of the tribological tests show that the values of the parameters under investigation (coefficient of friction and linear wear) were different from each other. Some similarity was observed between the evaluated level of wear characteristics after the friction process in the environment of human saliva and that in the environment of one of the mucins tested. Microscopic observations of the surfaces of the enamel samples after friction revealed varied forms of tribological wear.

3

Badania właściwości tribologicznych i fizykochemicznych śliny ludzkiej i jej substytutów

Andrysewicz E., Mystkowska J., Dąbrowski J. R., Krawczyk-Dembicka E.

Engineering of Biomaterials

|

2013

|

Vol. 16, no. 118

23--29

PL

W pracy przedstawiono wyniki badań in vitro wybranych właściwości fizykochemicznych i tribologicznych śliny ludzkiej, własnych preparatów i handlowych substytutów śliny dostępnych na polskim rynku. Oceniano wartość pH, lepkość dynamiczną oraz współczynnik tarcia i zużycie objętościowe pary tribologicznej (szkliwo-ceramika korundowa) testowanej w środowisku ślin. Otrzymane wyniki badań wykazały różnice pomiędzy testowanymi preparatami w zakresie przeprowadzonych badań. Najlepszym z własnych preparatów, pod kątem właściwości tribologicznych, był roztwór mucyn typ III w buforowanym roztworze soli fizjologicznej.

EN

In this work, the results of in vitro studies of selected physical, chemical, and tribological properties of human saliva, self-made preparations, and commercial saliva substitutes available on the Polish market, have been presented. The pH value, absolute viscosity, as well as the friction coefficient and volumetric wear of the tribological pair tested in the environments of saliva and saliva substitutes were evaluated. The obtained results of studies showed differences between the tested preparations in the scope of the evaluated parameters. The best among the self-made preparations, in terms of tribological properties, was a type III mucin solution in a buffered saline solution.

4

The influence of saliva and its substitutes on corrosion of some implant alloys

Andrysewicz E.

Acta Mechanica et Automatica

|

2013

|

Vol. 7, no. 2

69--74

EN

The purpose of this paper is evaluation of the influence of human saliva and its substitutes on the corrosion re sistance of some implant alloys used in stomatology, which included: austenitic steel (316L), titanium alloy (Ti6Al4V), and cobalt alloy (CoCrMo). Corrosion studies were conducted by means of the potentiodynamic method with the application of the VoltaLab 21 kit with VoltaMaster 4 software. The reference electrode was a saturated calomel electrode (SCE), whereas the counter electrode was a platinum electrode. The results of conducted studies indicate an increased current density in the passive range on potentiodynamic curves of studied alloys in the envi- ronment of human saliva, and also in a commercial saliva solution – Mucinox. On the basis of conducted corrosion studies, it can be stated that in terms of corrosion resistance the developed saliva substitutes may constitute competitive solutions to commercial saliva substitutes. The prepared substitutes should be studied further from the perspective of practical application for patients. The original value of the paper is a proposition of new saliva substitutes.

5

Influence of artificial saliva compositions on tribological characteristics of Ti-6Al-4V implant alloy

Andrysewicz E., Mystkowska J., Kolmas J., Jałbrzykowski M., Olchowik R., Dąbrowski J. R.

Acta of Bioengineering and Biomechanics

|

2012

|

Vol. 14, no. 4

71-79

EN

The present paper describes the results of tests on the influence of human saliva and its substitutes on tribological characteristics of implant materials on the example of the Ti-6Al-4V (a-Ti) titanium alloy. The saliva substitutes were prepared on the basis of pyrophosphates and mucins dissolved in saline buffer. The results of the presented tribological tests show that the values of the parameters under research varied from each other, while much similarity was observed between the evaluated level of wear characteristics after the friction process in the human saliva environment and that in the environment of one of the mucins tested. The microscopic observations of surfaces of the a-Ti samples after friction revealed varied forms of tribological wear. Infrared microspectroscopy studies of surfaces of the a-Ti samples after friction revealed the presence of secondary lubricating films based on mucin found in the artificial saliva solutions.