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1 2010

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Elektrochromatografia planarna w układzie zamkniętym

Płocharz P.W., Ślązak P., Hałka-Grysińska A., Chomicki A., Dzido T.H.

Wiadomości Chemiczne

2010

[Z] 64, 1-2

61-80

Planar electrochromatography in closed system (pressurized planar electrochromatography, PPEC) is the separation mode in which mobile phase is driven into movement by electroosmotic effect relative to adsorbent layer of the chromatographic plate. Solute band migration in the PPEC mode is governed by two effects: solute partition in a mobile phase - stationary phase system and electrophoresis when solutes undergo dissociation. Development of the methodology and equipment for PPEC are described in the paper. Attributes of the mode such as high separation efficiency and selectivity changes relative to liquid chromatography, especially thin-layer chromatography, are discussed. The mode is characterized by relatively short separation time and high performance of the separating system. Plate height approaches the value equal to two particle diameter of the stationary phase (adsorbent layer). Different separation selectivity mentioned is especially advantageous for application in two dimensional separation. The example of such separation of a test dye mixture, applying high performance thin layer chromatography in the first dimension and pressurized planar electrochromatography in the second one, is also presented and discussed in the paper. The mentioned advantages are very promising for development of the mode and its future application in laboratory practice. However, few main challenges concerned with production of chromatographic plates dedicated to the mode and construction of the equipment convenient for the operator are primary concern of future development of the mode.