Wyniki wyszukiwania - BazTech

Ograniczanie wyników

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: root formation

Sortuj według:

Ogranicz wyniki do:

Germination and In Vitro Propagation of Gundelia tournefontii as an Important Medicinal Plant

Shatnawi Mohamad, Majdalawi Majid, Shahrour Wesam, Abu-Zahra Taleb R., Al-Tawaha Abdel Rahman

Ecological Engineering & Environmental Technology

2022

Vol. 23, iss. 1

57--64

Medicinal plants are important sources of medical and many other pharmaceutical goods. The conventional propagation scheme is the main means of proliferation and takes a long period of time because of poor germination and also low clonal uniformity. Rapid shoot multiplication of Gundelia tournefontii was attained from meristems on Murashige and Skoog (MS) basal medium containing 6-Benzyladenine (BAP) or kinetin, with the adding of 30 g/L sucrose. The highest number of new microshoots per explant (9.2) was attained on the MS medium enhanced with BAP and 0.05 mg/L IBA. After 13 to 14 days, microhoots started rooting on the MS medium enhanced either Indole3-butyric acid (IBA), Indole-3-acetic acid (IAA), or Naphthalene acetic acid (NAA) with the addition of 30 g/L sucrose. Using 100 to 300 ppm Gibbreline GA3 resulted in an increase in germination percentage when soaking with GA3 for 12 hours. Gamma radiation had a significant effect on the growth of the in vitro explants. Gamma radiation had a significant effect on the germination percentage of G. tournefontii seeds. The in vitro propagation plant of G. tournefontii plants could be used for the marketable clonal propagation of this important species or for future studies.

Antimicrobial Activity and Micropropagation of Some Rare and Endangered Plants of Jordan

Shatnawi Mohamad, Abubaker Samih, Odat Nidal, Al-Tawaha Abdel Rahman, Majdalawi Majdi

Journal of Ecological Engineering

2021

Vol. 22, nr 6

151--158

The medicinal plants of Jordan are under threat due to several factors of habitat perturbation. Some of these factors include ruinous over-harvesting, climate change, competition and invasion. In this study, the authors employed a reliable method for micropropagation and assessment of antimicrobial activity of some important medicinal plants of Jordan. Seeds were surface sterilized and germinated on agar water media, and then shoots were cultured on Murashige and Skoog (MS) medium with the addition of 3% sucrose. In order to generate enough plant material, microshoots were transferred after fourteen days onto hormone free MS medium. Among all studied plants, it was found that the Achellia millefolium and Moring perigrina plants successfully multiplied in vitro containing different 6-benzyl amino purine (BA). For potential antimicrobial activity, the ex vitro (field leaf plants) and in vitro (plantlet) extracts were evaluated against some bacteria strains using ethanolic extracts. Both ex vitro and in vitro plants extracts showed the antimicrobial activity against the microorganism tested. The results from the study suggest that these two plants showed good antimicrobial activity against the different tested bacteria.