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Biological applications of electron spin resonance methods - spin trapping and spin labelling

Maliszewska-Mazur M.

Biocybernetics and Biomedical Engineering

2007

Vol. 27, no. 4

85-97

In this paper, use of electron spin resonance methods in biological investigations and trends in their development are presented. The first method, spin trapping technique consists in using of spin traps, i.e. compounds that form a stable free radical by reacting with an unstable free radical. Hydroxyl radical generation by living human granulocytes stimulated by dihydralazine was determined by ESR using DMPO as a spin trapping reagent. The second method, spin labelling is an example of a "reporter group" technique. The effect of dihydralazine on membrane bilayer was found by spin-labelling technique with the use of 5-, 12-, 16-doxylstearate.