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1

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

Çelebi B.

Acta Chromatographica

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2017

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Vol. 29, no. 2

143--159

EN

New polyacrylate-based monosized-porous polymer beads were proposed as a stationary phase for the separation of polar compounds by microbore reversed-phase chromatography. For this purpose, monosized-porous poly(glycerol dimethacrylate-co-glycerol-1,3-diglycerolate diacrylate), poly(GDMA-co-GDGDA), beads with hydroxyl functionality were synthesized by a modified seeded polymerization. The selected octadecylating agent, stearoyl chloride (SC), was covalently attached onto the hydrophilic beads via a direct, single stage reaction with a simple synthetic route. SC attached-poly( GDMA-co-GDGDA) beads were slurry-packed into the microbore columns and used as separation medium microbore reversed-phase chromatography. The stationary phase was used for separation of alkylbenzenes and polar analytes by micro reversed-phase chromatography, using mobile phases with low acetonitrile content. Theoretical plate number (TPN) values up to 12,000 plates m−1 and 10,000 plates m−1 for alkylbenzenes and polar analytes, respectively, were achieved. The results also showed that poly(GDMA-co-GDGDA) hydrogel beads are a promising starting material for a number of chromatographic applications like reversed-phase (RP) chromatography, hydrophilic interaction chromatography (HILIC), ion-exchange chromatography (IEC), and affinity chromatography with a single-stage surface modification.

2

Retention and separation efficiency of some synthetic oligopeptides in reversed-phase thin-layer chromatography

Gwarda R. Ł., Tomczyszyn A., Misicka A., Dzido T. H.

Acta Chromatographica

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2015

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Vol. 27, no. 1

1--14

EN

Influence of the following variables such as adsorbent type, type and concentration of organic modifier in mobile phase, type and concentration of ion-pairing reagent, or pH of the mobile phase buffer on retention of some synthetic peptides in reversed-phase high-performance thin-layer chromatography systems has been investigated. The investigations have been also focused on influence of the variables mentioned on solute zone shape regarding optimization of their separation. Remarks about solute retention mechanism have been also provided.

3

Analiza specjacyjna Cr(lll) i Cr(VI) w wodzie. Cz. III - możliwości i problemy rozdzielania form specjacyjnych chromu wysoko sprawną chromatografią cieczową

Walkowiak G., Sęk K., Barałkiewicz D.

Analityka : nauka i praktyka

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2010

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nr 2

32-35

PL

Formy specjacyjne chromu można rozdzielać i oznaczać na wiele sposobów. Wśród metod rozdzielania najpowszechniej stosowana jest wysoko sprawna chromatografia cieczowa HPLC, ze względu na dostępność różnorodnych mechanizmów rozdzielania oraz faz ruchomych, pozwalających na zachowanie oznaczanej formy w stanach niezmienionych.

4

Techniki chromatograficzne stosowane w oczyszczaniu białek. Cz. 1, Przegląd metod chromatograficznych

Błaszczyk U.

Laboratorium - Przegląd Ogólnopolski

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2009

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nr 9

50--54

PL

Cząsteczki białkowe mogą być rozdzielane na podstawie różnic w ich specyficznych właściwościach, takich jak: ładunek, rozmiar, hydrofobowość lub zdolność do swoistego wiązania określonych ligandów. Artykuł prezentuje krótki przegląd metod chromatograficznych stosowanych w procesie oczyszczania białek.

EN

Protein molecules can be separated on the basis of differences in their specific properties such as charge, size, hydrophobicity or their ability to bind specific ligands. The article presents a brief overview of chromatographic methods used in the process of protein purification.