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Evaluation of the impact of decellularization and sterilization on tensile strength transgenic porcinedermal dressings

Joszko Kamil, Gzik-Zroska Βożena, Kowalewska Edyta, Klama-Baryła Agnieszka, Suchoń Sławomir, Burkacki Michał, Wolański Wojciech

Acta of Bioengineering and Biomechanics

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2019

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Vol. 21, no. 3

87--97

EN

The aim of this paper was to evaluate which method of acellularization and sterilization is optimal, in the meaning of which processes have the least impact on the deterioration of mechanical properties of porcine tissues used for xenogeneic applications. Methods: The static tensile probe was conducted for 80 skin specimens obtained from transgenic swine, which are used as a wound dressing for skin recipient. Obtained data were subsequently analyzed with the use of statistical methods. Results: It was found that Young’s modulus for the samples after the sterilization process for the dispase substance and the mixed method (SDS + trypsin) were statistically significantly changed. In the case of dispase, Young’s modulus value before the sterilization process was 12.4 MPa and after the value increased to 28.0 MPa. For the mixed method (SDS + trypsin) before the sterilization process Young’s modulus value was 5.6 MPa and after it was increased to 6.3 MPa. The mixed method (SDS + trypsin) had the slightest effect on changing the mechanical properties of the samples before and after the sterilization process. Conclusions: It was confirmed that different methods of acellularization and the process of sterilization have an influence on the change of mechanical properties of the skin of transgenic swine. In the authors’ opinion, the mixed method (SDS + trypsin) should be recommended as the best one for the preparation of transgenic porcine dermal dressings because it ensures a smaller probability of dressing’s damage during a surgical procedure.

2

Gait changes in patients after reconstruction of facial bones with fibula and iliac crest free vascularized flaps

Syczewska M., Krajewski R., Szczerbik E., Kalinowska M.

Acta of Bioengineering and Biomechanics

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2018

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Vol. 20, no. 1

185--190

EN

Patients with malignant tumours of the oral cavity require its surgical removal and reconstruction of the bone and soft tissues. The grafts are obtained either from leg (fibula) or pelvis (iliac crest). The removal of grafts from the locomotor apparatus can impair the gait. The aim of this study was to find out how the localization of donor site influences the gait pattern. Methods: Results obtained for 30 patients were analyzed (16 fibula graft, 14 iliac crest graft). Patients underwent instrumented gait analyses three times (VICON system): before surgery, 2–4 month after the surgery, and 4–8 months after the surgery. Results: In both groups several gait parameters were changed. Two parameters changed in both groups: gait speed and cadence. In patients receiving iliac crest graft the changed gait variables were: pelvic rotation, hip range in sagittal plane (operated side), knee range in sagittal plane (operated side), foot dorsiflexion in swing on both sides. In patients receiving fibula flap the changed gait variables were: tilt, range motion of the tilt, minimum hip flexion (operated side), time to maximum knee flexion (non-operated side), GGI (non-operated side) and step length (nonoperated side). Conclusions: The primary gait deviations occurring after surgery, and the compensatory mechanisms which subsequently arise depend on the localization of graft donor site. The results indicate that the patients in whom fibula flap was used have less problems with gait pattern after the surgery than the patients receiving iliac crest graft.

3

Zastosowanie metody laserowej przepływometrii dopplerowskiej do oceny ukrwienia zespolenia przełykowego u chorych poddawanych operacji rekonstrukcji przełyku

Przywara S., Wallner G., Dąbrowski A., Terlecki P., Zubilewicz T., Michalak J., Wroński J.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

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2005

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Vol. 11, nr 3-4

32-34

PL

W pracy omówiono wykorzystanie metody laserowej przepływometrii dopplerowskiej do oceny ukrwienia zespolenia przełykowego u chorych poddawanych operacji rekonstrukcji przełyku. Metoda laserowej przepływometrii dopplerowskiej umożliwia śródoperacyjną, obiektywną ocenę przepływu krwi w mikrokrążeniu okolicy zespolenia przełykowego, a także w samym substytucie formowanym, w celu zastąpienia patologicznie zmienionego odcinka przełyku. Jej rutynowe wykorzystanie może zmniejszać prawdopodobieństwo wystąpienia powikłań pooperacyjnych w postaci przetok w okolicy zespolenia czy zwężeń.

EN

The paper presents application of laser Doppler flowmetry in the assessment of microcirculatory perfusion in the area of esophageal anastomosis in patients undergoing reconstructive surgery of esophagus. The method enables objective evaluation of blood flow in microcirculation of anastomosis area as well as In the substitute used for reconstruction. Routine measurements with laser Doppler flowmetry may diminish the rate of postoperative complications such as anastomosis leakage or stenosis.

4

Problematyka rekonstrukcji dużych ubytków tchawicy materiałami alloplastycznymi

Ścierski W., Namysłowski G., Błażewicz M., Pilch J.

Inżynieria Biomateriałów

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2003

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R. 6, nr 26

27-33

PL

Do chwili obecnej nie wynaleziono odpowiedniego materiału alloplastycznego, którego zastosowanie w chirurgii rekonstrukcyjnej dużych segmentowych ubytków tchawicy dawałoby dobre długotrwałe wyniki w badaniach doświadczalnych. Głównym problemem są ponowne zwężenia (powstające przede wszystkim w związku z brakiem urzęsionego nabłonka na wewnętrznej powierzchni protezy), odrzucenia całego przeszczepu, lokalne i infekcje i krwotoki. Wciąż prowadzone są badania, których celem jest wytworzenie materiału o odpowiednich właściwościach fizykochemicznych oraz umożliwiającym migrację komórek i pokrycie wewnętrznej powierzchni nabłonkiem oddechowym. W artykule przedstawiono ogólny stan wiedzy na temat rekonstrukcji dużych ubytków tchawicy oraz przegląd literatury dotyczący zastosowania materiałów alloplastycznych w badaniach doświadczalnych na zwierzętach.

EN

There is no appropriate alloplastic material developed to date, the use of which in reconstructive surgery of large segment trachea defects would bring about positive long-term experimental results. The principal problems are related to re-stenosis (due to lack of ciliated epithelium on the inside surface of the prosthesis), total rejection of graft, local injection and bleedings. Intensive investigations are still being carried out, focused mainly on formulation of the material with appropriate physico-chemical properties allowing for migration of cells and covering of the inside surface by respiratory epithelium. This paper presents current state of knowledge on reconstruction of large tracheal defects, as well as the review of available literature relative to application of alloplastic materials in experimental works on animals.