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HPLC monitoring of the microsomal stability of rutin and quercetin

Szőke É, Petroianu G., Tekes K., Benkő B, Szegi P., Laufer R., Veress G

Acta Chromatographica

2009

Vol. 21, no. 3

399--410

Reversed-phase HPLC has been used to monitor the concentration of the two major Chamomile components rutin and quercetin during rat liver microsomal treatment. The possibility of microsomal oxidative metabolism or stability of these two components was examined using a guard-column without any clean-up. The concentration of quercetin decreased when exposed to rat liver microsomal media whereas the rutin concentration did not change significantly over one hour of treatment.