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EN
Using the extended Huygens–Fresnel principle and Rayleigh scattering theory, optical trapping of the low index of refraction particles using a focused Gaussian Schell-model (GSM) non-vortex beam, a focused GSM vortex beam, and two face-to-face focused GSM vortex beams have been studied. The results demonstrate that the focused GSM non-vortex beam cannot capture the low index of refraction particles, however, the focused GSM vortex beam can be a two-dimensional trap of particles in the z-axis, and the transverse gradient force Fgrad,x and the trapping equilibrium region increase as the topological charge m increases. As the focal length f or the refractive index of particles np decreases, the radiation forces increase and the trapping ability also enhances. To trap the low index particles in three-dimensional space, we adopt that the two face-to-face focused GSM vortex beams can be used to construct an optical potential well. The transverse gradient force of two face-to-face focused GSM vortex beams is twice that of a single GSM vortex beam. The limit of the radius for the low index of refraction particles that were stably captured has also been determined. The obtained results provide valuable information for trapping and manipulating the low index of refraction particles using GSM vortex beams, which may be applied in micromanipulation, biotechnology, nanotechnology and so on.
EN
Streaming velocity depends on intensity and absorption of ultrasound in the media. In some cases, such as ultrasound scattered on blood cells at high frequencies, or the presence of ultrasound contrast agents, scattering affects the streaming speed. The velocities of acoustic streaming in a blood-mimicking starch suspension in water and Bracco BR14 contrast agent were measured. The source of the streaming was a plane 20MHz ultrasonic transducer. Velocity was estimated from the averaged Doppler spectrum. The single particle driving force was calculated as the integral of the momentum density tensor components. For different starch concentrations, the streaming velocity increased from 8.9 to 12.5mm/s. This corresponds to a constant 14% velocity increase for a 1 g/l increase in starch concentration. For BR14, the streaming velocity remained constant at 7.2mm/s and was independent of the microbubbles concentration. The velocity was less than in reference, within 0.5mm/s measurement error. Theoretical calculations showed a 16% increase in streaming velocity for 1 g/l starch concentration rise, very similar to the experimental results. The theory has also shown the ability to reduce the streaming velocity by low-density scatterers, as was experimentally proved using the BR14 contrast agent.
EN
Imaging the tissue displacements caused by Acoustic Radiation Force Impulse (ARFI) provides qualitative tissue elasticity maps around the focus. To increase imaging range, multi-focus techniques combine several images obtained with different focal depths. Since the acoustic radiation force depends on focus depth, axial distance and steering angle, a normalization process is required before blending multi-focal ARFI images so that changes in the displayed displacements represent true tissue elasticity variations. This work analyzes the sources of displacement variability in multi-focal-zone ARFI and proposes a procedure to normalize and combine partial images. The proposal is based on the system focal configuration, transducer characteristics and global tissue parameters found by ultrasonic measurements. Performance of the proposed algorithm is experimentally evaluated with tissue mimicking phantoms.
EN
The aim of this work was to use the streaming phenomena to assist clot dissolution in blood vessel. Such treatment is called sonothrombolysis. Acoustic streaming is a steady flow in a fluid driven by the acoustic wave propagating in a lossy medium. It is a non-linear effect and it depends on ultrasound intensity, and sound absorption in the media. The source of ultrasound was a flat piezoceramic disc generating long pulses at 1 MHz frequency and 0.2 W/cm2 ITA acoustical intensity. The streaming was generated in a vessel simulating free space, and next repeated in a multi-well cell culture plate, and in the limited space inside the 8 mm diameter silicone tube positioned perpendicular to the ultrasonic beam. The tube was filled with a mixture of water, glycerol, and starch, so with acoustic properties similar to blood. The streaming velocity was recorded either by the Siemens Acuson Antares ultrasonic scanner operating in the color Doppler mode at 8.9 MHz, or by the custom built 20 MHz pulsed Doppler flowmeter. The results obtained using both systems were very similar. The recorded streaming velocities were 3.2 cm/s, 6.1 cm/s and 0.3 cm/s, respectively. They were an order of magnitude smaller than that calculated theoretically. However, the results obtained confirm existence of streaming, even very close to the source, in the limited space. This effect will be explored in in-vitro experiments of blood clot dissolution within the tube simulating a blood vessel.
EN
The analysis of streaming caused by aperiodic sound of different types (switched on at transducer sound or sound determined by initial conditions) is undertaken. The analysis bases on analytical governing equation for streaming Eulerian velocity, which is a result of decomposition of the hydrodynamic equations into acoustic and non-acoustic parts. Its driving force (of acoustic nature) represents a sum of two terms; one is the classic one, which, being averaged over the sound period, coincides with the well-known expression. The second one depends on the periodicity of the sound; at the axis of beam propagation it becomes exactly zero after averaging for the strictly periodic sound but differs from zero for other acoustic waves. Both terms are nonlinear and proportional to the standard attenuation due to shear, bulk viscosities and thermal conductivity. Numerical analysis reveals a qualitative agreement with experimental data. Some theoretical conclusions concerning features of streaming caused by sound determined by initial conditions, are made.
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