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Phylogenetic Analysis and Screening of Hydrolytic Bacteria with Hydrolase Enzyme Activity from Hospital Wastewater of Semarang, Central Java, Indonesia

Ethica Stalis Norma, Sabdono Agus

Ecological Engineering & Environmental Technology

2021

Vol. 22, iss. 2

81--86

Hydrolytic bacteria have the ability to convert organic material which is very useful in the bioremediation of biomedical waste. The objective of the study was to investigate the hydrolytic bacterial diversity that has the hydrolase enzyme activity. The medical waste sampling was carried out at Roemani Hospital and KRMT Wongsonegoro Hospital, Semarang Central Java in mid-August 2016. Microbiological and molecular studies, covering isolation and purification, hydrolase productivity tests, and bacterial determination, were conducted at the Tropical Marine Biotechnology Laboratory, Faculty of Fisheries and Science, UNDIP. The 16S rDNA gene sequence analysis was carried out by using the BLAST GeneBank NCBI homology test, while the phylogenetic tree construction was carried out by means of Clustal X software and Phylegenetic analysis using parsimony (PAUP vers 4.0). The test results showed that 26 hydrolytic bacterial isolates were able to produce four types of extracellular hydrolase enzymes. The results of the enzyme production test showed that there were only 2 bacterial isolates capable of producing the four types of enzymes. Microbiological and molecular polyphasic identifications showed that the 2 isolates, R1-17, and R2-6 isolates are closely related to Virgibacillus salarius strain B-11 and Bacillus subtilis strain VITVB1, respectively. Even though there are only 2 isolates capable of producing the 4 types of extracellular enzymes, it seems that hospital biomedical waste is a potential source for obtaining hydrolytic bacteria.

First Report on Fusarium brachygibbosum Isolate FIR 16_ITS Isolated from Iraqi Wheat Plant

Ali Hamdia Z., Hameed Mahdi S., Abdulrahman Abdulrahman A., Saood Hutham M.

Journal of Ecological Engineering

2020

Vol. 21, nr 3

81--86

In this study, the Fusarium Brachygibbosum fungus isolate FIR 16_ITS was isolated for the first time from the infected wheat plants in Iraq, during the winter of 2018, in the AL-Tewatha region – 20 km south east of Baghdad. In order to verify the identity of the species of the F. brachygibbosum isolate FIR 16_ITS with the growth symptoms on the wheat plants, the wheat seeds and tissue plants were obtained and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique is important in verifying the species of F. brachygibbosum isolate FIR 16_ITS. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. The authors successfully obtained the amplified products of the ITS-rDNA region, and a sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the F. brachygibbosum species. It was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).