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1

Multiresidue determination of antibiotics in preserved eggs using a QuEChERS-based procedure by ultrahigh-performance liquid chromatography tandem mass spectrometry

Li Y., Chen Z., Wen S., Hou X., Zhang R., Ma M.

Acta Chromatographica

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2018

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Vol. 30, no. 1

9--16

EN

A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) were optimized and validated for 16 antibiotics belonging to three families (macrolides, quinolones, and sulfonamides) that were found in preserved eggs. Samples were extracted in 4 mL water and 10 mL acetonitrile with 1% acetic acid and subjected to a cleanup procedure using dispersive solid-phase extraction with C18 and primary secondary amine sorbents, prior to detection by UHPLC–MS/MS. Matrix-matched calibration was used for quantification to reduce the matrix effect with limits of quantification in the range of 0.3–3.0 μg/kg. Validation of the method was conducted by recovery and precision experiments. Recoveries of the spiked samples ranged from 73.8% to 127.4%, and the intra- and inter-day relative standard deviations were lower than 21.2% and 22.3%, respectively. This method was successfully applied to the analysis of antibiotics in preserved egg samples.

2

Ból jako działanie niepożądane : dolegliwości bólowe w trakcie farmakoterapii wybranymi grupami leków

Baranowski J., Klęczar K., Białecka M.

Problemy Nauk Stosowanych

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2017

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T. 6

107--118

PL

Wstęp i cel pracy: Farmakoterapia jest podstawą dzisiejszej medycyny. Wszystkie grupy leków obarczone są jednak ryzykiem wystąpienia działań niepożądanych. Celem niniejszej pracy jest przedstawienie dolegliwości bólowych u pacjentów, wyindukowanych często stosowanymi grupami leków. Materiał i metody: Na podstawie dostępnego piśmiennictwa wskazano grupy leków, które mogą powodować działania niepożądane w postaci bólu. Ponadto podjęto próbę opisania patomechanizmu, występujących objawów klinicznych oraz możliwości terapeutycznych. Wyniki: Nadużywanie wszystkich leków przeciwbólowych i terapia nitratami często prowadzi do polekowych bólów głowy. Opioidy mogą indukować różnorodne dolegliwości bólowe, w mechanizmach: hiperalgezji indukowanej opioidami, tolerancji na opioidy, allodynii oraz bólu z odstawienia. Zarówno statyny jak i chinolony wywołują dolegliwości bólowe z układu ruchu. Chemioterapia jest przyczyną neuropatii obwodowej. Wnioski: Każda grupa leków jest obarczona działaniami niepożądanymi, które należy zgłaszać do stosownej instytucji.

EN

Introduction and aim: Pharmacotherapy is the core of modern medicine. Unfortunately all drugs may lead to side effects. The aim of this paper is to present the appearance of pain among patients undergoing a treatment with commonly used medications. Materials and methods: On the grounds of the available literature, the authors identify several drug groups that can cause pain. Moreover, pathomechanisms as well as clinical symptoms and possible therapies were described. Results: Headaches may be caused by a medication-overuse in the therapy with analgesics and nitrates. Opioids are known to induce pain in various mechanisms: opioid-induced hyperalgesia, tolerance, allodynia and withdrawal. Both statins and quinolones may lead to muscle ache. Peripheral neuropathy is a result of chemotherapy. Conclusion: None of the investigated drugs are free from side effects. The occurrence of such phenomena must be reported to the adequate institution.

3

Determination of quinolonones in food of animal origin by liquid chromatography coupled with fluorescence and mass spectrometric detection

Stoilova N., Surleva A., Stoev G.

Acta Chromatographica

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2014

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Vol. 26, no. 4

599--614

EN

High-performance liquid chromatography coupled with fluorescence (HPLC-FD) and tandem mass spectrometric detection (LC-MS/MS) was studied as a versatile tool for fast and reliable determination of nine regulated quinolones in food of animal origin (Council Regulation 2377/90/ECC). The sample pre-treatment protocol includes double step extraction with acetonitrile followed by solid phase extraction (SPE) cleanup on hydrophobic-lipophilic balance (HLB) cartridge. The separation of quinolones in HPLC-FD determination was performed on C18 Zorbax column with a gradient mixture of aqueous formic acid, methanol, and acetonitrile. A multi-wavelength excitation/emission program was used for sensitive quinolones detection. The separation efficiency of newly available chromatographic columns: Gemini C18 and Synergi Polar RP (fully porous particles), as well as Kinetex PFP and Poroshell 120 EC-C18 (core-shell particles), was studied in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis. Appropriate gradient elution program was designed for each column. Multiple reaction monitoring was used for selective determination of each quinolone. LC-MS/MS allowed quinolones determination in less than 5 min. Both methods showed detection limits below maximum residue limits for quinolones residues in food commodities.

4

Potentiometric determination of pKa values of some quinolones in methanol-water and acetonitrile-water binary mixtures

Sardohan T., Kir E.

Chemia Analityczna

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2009

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Vol. 54, No. 4

629-643

EN

pKa values of three quinolone compounds (ciprotloxacin, norfloxacin and enoxacin) in methanol—water (MeOH—water) and acetonitrile-water (ACN—water) mixtures were determined in potentiometric measurements. Standardization of the electrode system in MeOH-water and ACN-water mixtures was carried out potentiometncally by Gran's method. The obtained results were compared to the literature pKa values determined by various techniques and to the values predicted by the SPARC on-line pK 6calculator.

PL

Wyznaczono metodą potencjometryczną wartości pKa trzech związków chinolonowych: ciprofloksacyny, norfloksacyny i enoksacyny. Pomiary wykonywano w roztworach: meta-nol-woda i acetonitryl-woda. Wzorcowanie układu elektrod w układach: MeOH-woda i ACN-woda wykonano potencjometrycznie metodą Grana. Wyniki porównano z literaturowymi wartościami pK awyznaczonymi za pomocą różnych metod jak również z wartościami obliczonymi za pomocą programu SPARC.