Storage of medicinal plants may cause deterioration of the active principles with time, thus, reducing the efficacy of plants. Therefore, quantification of the active principle is essential before using the crude drug. Phyllanthus amarus (PA) contains lignans, namely, phyllanthin and hypophyllanthin, which shows anti-hepatotoxic activity. In this paper, we highlight the effect of storage conditions on the quantification of bioactive markers by high-performance liquid chromatography (HPLC) analysis in the crude plant material of PA. HPLC analysis of crude PA samples stored for certain period at long-term study (LS, 30 °C and 65% RH), accelerated study (AS, 40°C and 75% RH), and real-time study (RT) conditions was carried out using the LiChroCART Purospher® STAR RP-18 endcapped (250 × 4.6 mm, 5 μm) column along with a Purospher STAR RP 18e (4.0 × 4.0 mm, 5 μm) guard column using methanol:water (70:30) at a flow rate of 0.7 mL min−1 with ultraviolet (UV) detection at 220 nm. The HPLC study indicated that PA samples kept under LS condition are rich in lignan contents as compared to the samples stored under AS and RT study conditions. Therefore, PA should be used fresh to get maximum concentration of active lignans or it should be stored under LS conditions up to 6 months.
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Atykłułprzedstawia zagadnienia ilościowej oceny użytkowej przyrządu pomiarowego. Podano usutułowanie jakości w teorii i praktyce oraz podstawowe definicje. Przdstawiono metodę oceny jakości bazującą na wiedzy użytkownika, wzorowana na metodach inżynierii wiedzy. Metoda korzysta z zaawansowanych procedur statystycznych do obiektywizowania wyników badań. Uwzględnia podejście heurystyczne i podejście algorytmiczne.
EN
In the paper, issues of the quantitative estimation of the functional quality of the gauge are presented. The location of the quality in the theory and the practice and basic definitions in given. Method of the quality estimation based on the user knowledge, modelled on methods of the knowledge engineering is presented. Presented method use advantage of advanced statistical procedures to objectivating of results of resarch. Both the heuristic approach and the algorithmic approach are taken into acount.
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