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A novel HPLC method for simultaneous quantification of eleven phenolic antioxidants in commercial personal care and food products which contain extracts of green apple, pomegranate and argan oil

Moussad Ranim I., Salam Randa A. Abdel, Hadad Ghada M., EL Gindy Alaa, Foudah Ahmed I., Hameed Eman A. Abdel

Acta Chromatographica

2022

Vol. 34, no. 1

77--86

In this study, a simple, fully validated and rapid reversed-phase HPLC with photodiode array detector method was developed for the simultaneous determination of 11 selected phenolic antioxidants over 33 min in personal care and food samples containing extracts of green apple, pomegranate (Ponica granatum) and argan oil (Argania spinosa). The method was performed using NUCLEODUR C18 column 5 µm particle size and 12.5 cm length. The HPLC mobile phase was prepared as follows, solution A: 1% aqueous acetic acid and solution B: Acetonitrile. The method was gradient at flow rate 1.0 mL/min with a simple mobile phase, minimal sample preparation, and diminished organic solvent usage (3% acetonitrile for almost 90% of the run time). The detection was carried out at 278 nm. The method presented good precision and accuracy with RSD% values ranged between 0.33 and 1.94% and wide linear range. The developed method was successfully applied on 67 personal care and food products present in Egyptian market and can be used for routine screening in laboratory for the regular quality control of the antioxidant content for products containing the mentioned extracts.

Matrix solid-phase dispersion method coupled with high-performance liquid chromatography for the simultaneous quantification of selected compounds from freeze dry pomegranate juice

Kamal Y. T., Yusufoglu H., Alam P., Fouda A.

Acta Chromatographica

2018

Vol. 30, no. 3

153--157

In this research, a novel method was developed for the matrix solid phase dispersion (MSPD) followed by high-performance liquid chromatography (HPLC) quantification of four marker constituents (vitamin C, gallic acid, rutin, and ellagic acid) in the freeze-dried pomegranate fruit juice. Various MSPD parameters like type of dispersant, sample–dispersant ratio, solvents, its volume, and time of extraction have been optimized after many trials. Furthermore, HPLC method has been developed and optimized for the analysis of all four components. The HPLC separation was achieved using a 250 × 4.6 mm column, particle size of 5 μm, C18 reverse phase column, with a mobile phase consisting of acetonitrile and 0.05% H3PO4, in gradient elution mode with a mobile phase flow rate of 1 mL/min, using ultraviolet (UV)–visible detection at 254 nm. All calibration curves showed good linear regression (r2 ≥ 0.9925) within test ranges. The extraction recoveries of the marker constituents analyzed by MSPD methods were found as ranging from 97.5% to 103.5%. From comparing the chromatograms, validation data and other parameters like time, labor, and feasibility, we found that MSPD technique was most suitable for the analysis as compared to conventional liquid–liquid extraction technique.