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Culture of human autologous chondrocytes on polysulphonic membrane - preliminary studies

Płończak M., Czubak J., Chwojnowski A., Kupikowska-Stobba B.

Biocybernetics and Biomedical Engineering

2012

Vol. 32, no. 3

63-67

This work investigated an effective method of isolation and culture of human autologous chondrocytes placed on a polysulphonic membrane. The cartilage was taken from the hip joint of 78 years old woman who underwent total hip ar throplasty due to idiopatic arthrosis and from the knee of 46 years old man with cartilage lesion from non-weight bear area. The cells were released from the matrix in the course of enzymatic digestion. The isolated cells were placed with parts of polysulphonic membrane in the same culture flask and incubated. Due to evaluation the weight of tissue grown on the polysulphonic membrane the elementary analysis was performed. The elementary analysis of the polysulphonic membrane slices after ten weeks of the culture revealed higher concentration of the tissue on one part of the membrane in case of the older woman - 0.726 mg of protein per 1 mg of the membrane then in case of man - 0.513 mg per 1 mg. The established method of isolation and culture of chondrocytes is effective enough to provide a sufficient number of cells that can be used as a transplant.

Repair of articular cartilage full thickness defects with cultured chondrocytes placed on polysulphonic membrane - experimental studies in rabbits

Płończak M., Czubak J., Hoser G., Chwojnowski A., Kawiak J., Dudziński K., Czumińska K.

Biocybernetics and Biomedical Engineering

2008

Vol. 28, no. 2

87-93

Autologous osteochondral transplantation is one of the methods. that can be used to create hyaline or hyaline - like repair in the defect area. The purpose of the present study was to repair the full - thickness articular cartilage defects in nine rabbits' knee joints with autologous cultured chondrocytes. An articular cartilage defect was created on the patellar groove of the femur. The defect was filled with the chondrocytes cultured in vitro and placed into the knee on a polysulphonic membrane. 8 weeks after the operation the reparative tissue was analyzed macroscopically and histologically. The surfaces of the reparative tissue were smooth, and the defects were filled with mature hyaline cartilage in five cases. In two cases the reparative hyaline cartilage was immature and there was worse integration of the grafted tissue into the adjacent normal cartilage. The surface of the grafted area was irregular, the reparative tissue was desintegrated and incompletely differentiated. The results suggest that transplantation of the autologous chondrocytes cultured in vitro and placed into the knee on the polysulphonic membrane is effective in repairing of the articular cartilage defect.