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Enzyme electrodes constructed on basis of oxygen electrode with oxidases immobilised by sol-gel technique

Przybyt M., Białkowska B.

Materials Science

2002

Vol. 20, No. 2

63-79

In this work, results of immobilisation of enzymes in silica gel on oxygen electrode are reported. As the model enzyme glucose oxidase was used. The influence of the composition of the casting solution (gel precursor, pH of the enzyme solution, sol-buffer ratio) on the electrode response was investigated. Also, the addition of ?-aminopropyltriethoxy silane to the casting solution was checked. The best electrode with stable signal was obtained, when the formed gel was not very rigid (buffer, pH 6 or 7 and high buffer-sol ratio). For the optimal composition of the casting solution, some properties of the glucose electrode were investigated (stability, pH profile and influence of temperature). The method of sol-gel entrappment was also used to obtain the electrodes sensitive for disaccharides by coimmobilisation of invertase, lactase and maltase with glucose oxidase. In addition, preliminary results for other oxidases immobilised by this method are presented.