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2 Optica Applicata

2 2011

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The low temperature co-fired ceramics (LTCC) chip for polymerase chain reaction (PCR) application

Bembnowicz P., Herbut P., Małodobra M., Karpiewska A., Golonka L. J., Jonkisz A., Dobosz T.

Optica Applicata

2011

Vol. 41, nr 2

471--480

The DNA (deoxyribonucleic acid) amplification chip made of DP951 (from DuPont) ceramics consists of a microchamber, internal and external metallization. The external metallization is used for attachment of SMD (surface mount device) electronics elements. The internal metal layer improves thermal conditions inside the chamber. The temperature distribution in the chamber is verified by numerical simulation. Heating is realized by an external SMD resistor. The temperature measurements are made by attached Pt100 component. The temperature control is realized by a microcontroller based electronic system. Settings and visualization of the polymerase chain reaction (PCR) process parameters are actualized by dedicated PC (personal computer) software. The system is successfully tested. The DNA amplification inside the low temperature co-fired ceramics (LTCC) chip is achieved.

Low temperature co-fired ceramics (LTCC) microsystems

Golonka L., Bembnowicz P., Jurkow D., Malecha K., Roguszczak R., Tadaszak R.

Optica Applicata

2011

Vol. 41, nr 2

383--388

The paper presents general information on ceramic microsystems. Low temperature co-fired ceramics (LTCC) technology is described in detail. Research and development on the LTCC sensors and microsystems carried out at Wrocław University of Technology (Poland) are presented. Microfluidic system, polymerase chain reaction (PCR) microreactor, acceleration sensor and sol-gel fiber optics for ceramic microsystems are described.