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Endothelial cells on pet vascular prostheses impregnated with polyester-based copolymers and coated with cell-adhesive protein assemblies

Chlupac J., Filova E., Riedel T., Brynda E., Pamuła E., Lisa V., Bacakova L.

Engineering of Biomaterials

2008

Vol. 11, no. 81-84

108--111

Arterial bypass surgery with synthetic vascular prostheses achieves poor patency rates compared to autogenous natural materials, and this is a challenge for tissue engineering research concerning small caliber vascular grafts. Modifications of the prosthetic surface followed by endothelial cell seeding may reduce thrombogenicity and intimal hyperplasia. Planar polyethylene terephthalate (PET) vascular prosthetic samples were impregnated with the copolymer poly(glycolide-L-lactide) (PGL) or with the terpolymer poly(glycolide-L-lactide-(e)caprolactone) (PGLCap) in order to lower the permeability of the knitted fabrics and ensure a less adhesive background. Subsequent modification with adhesive protein assemblies composed of collagen type I (Co) in conjunction with laminin (LM), fibronectin (FN) or fibrin (Fb) gel was performed to enhance cell adhesion. Bovine pulmonary artery endothelial cells (EC) of the CPAE line were seeded on to the coatings and subjected to static tissue culture conditions for 7 days. Impregnation of the PET prostheses decreased the initial adhesion and proliferation of the EC. After coating with the protein assemblies, the impregnated PET provided better substrates for cell culture than the protein-coated PET, on which the EC population started decreasing after 4 days of culture. The cells proliferated better on the CoFN, CoFb and CoFbFN coatings than on the Co and CoLM coatings. Impregnation type and adhesive matrix protein deposition may play an important role in successful endothelialization, healing and clinical performance of vascular grafts.

PET w butelkach barierowych

Piecyk L.

Laboratorium - Przegląd Ogólnopolski

2006

nr 12

26--29

Politereftalan etylenu (PET) jest tworzywem sztucznym o największej dynamice sprzedaży w skali globalnej. W niniejszym artykule przedstawiono zastosowanie PET w produkcji opakowań na napoje, w których ważną rolę odgrywają barierowe cechy materiału.

Polyethylene terephthalate (PET) belongs to plastics with the biggest dynamics of sales on global scale. In the paper PET's employment in the production of beverage packaging, where barrier features of material play an important role, is described.