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EN
he usability of the micellar enhanced ultrafiltration (MEUF) for removal of phosphates from aqueous solutions has been evaluated. The process was performed with the use of polyethersulfone membranes under various transmembrane pressures (0.1, 0.15, and 0.2 MPa). Phosphorous solutions containing 15 mg of PO3-4/dm3 and two cationic surfactants: octadecylamine acetate (ODA) and hexadecylpyridium chloride (CPC) were used in the tests. The concentration of ODA and CPC amounted to 2 and 3 CMC (Critical Micelle Concentration) as well as 2, 3 and 6 CMC, respectively. The results of the study showed that the surfactant type and its concentration influenced the process efficiency. The phosphate removal with the use of ODA was approximately 95%, whereas application of CPC gave unsatisfactory results.
EN
The this paper method of obtaining broad-pore membranes for application as scaffolds for chondrocyte cultivation is described. These membranes were obtained from polyethersulfone. They are characterized by the presence of both macropores of relatively large diameter, as well as micropores. These membranes are intended first of all for use in cultivation of the cartilage cells (chondrocytes). The membranes are obtained by the phase inversion method followed by dissolving cellulose present in the membrane. Cellulose is a macropore precursor. Cellulose is dissolved by means of a copper hydroxide ammonia complex. The membranes obtained are not cyto-toxic. The culture of chondrocytes derived from White New Zeeland breed rabbits developed very well on these membranes. The cell cultures were studied by observation under an optical microscope and scanning electron microscope. The protein mass increase on the membrane was determined by flame analysis. The results of these experiments did not show any negative effects of the membranes on the cell culture. Just the opposite, the cartilage cells development on the membranes proceeded very well. The results obtained show that the membrane developed is a very good scaffold for cell cultivation.
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