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Semi-empirical equation for the prediction of retention in normal phase liquid chromatography (NP-LC) with polar bonded stationary phases

Zapała W., Zapała L.

Ars Separatoria Acta

2008

No. 6

15-30

A semi-empirical heterogeneous adsorption model for the accurate prediction of analyte retention in a column chromatography with binary mobile phase on polar bonded adsorbent surface was proposed. The performance of the proposed equation was compared with two retention models reported in the literature. All models were verified for different LC systems by means of four criteria: the sum of squared differences between the experimental and theoretical data, approximation of standard deviation, Fisher test and F-test ratio.