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Point-of-Care Testing : Biosensor for Norepinephrine Determination

Baluta Sylwia, Swist Agnieszka, Cabaj Joanna, Malecha Karol

International Journal of Electronics and Telecommunications

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2020

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Vol. 66, No. 2

369--372

EN

An useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)- 9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with thin polymeric film. This sensing arrangement utilized the catalytic oxidation of NE to norepinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of enzyme – laccase. With the optimized conditions, the analytical performance demonstrated selectivity in a wide linear range (0.1–200x10-6 M) with a detection limit of 240 nM and a quantification limit of 365 nM. Moreover, the method was successfully applied for selective NE determination in the presence of interfering substances.

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Veterinary and medical lab-on-a-chip : based point of care diagnostic devices with optical detection

Walczak R.

Elektronika : konstrukcje, technologie, zastosowania

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2011

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Vol. 52, nr 3

44-46

EN

In this paper a brief review of lab-on-a-chip (LOC) based point-of-care devices utilizing fluorometric readout is given. Presented solutions were developed under European and national projects. Novel method and instrumentation of fluorescence readout is described. Three examples of veterinary and medical applicalions of LOC - all utilizing the same fluorometric readout idea - are briefly presented.

PL

W artykule przedstawiono krótki przegląd urządzeń typu point-of-care wykorzystujących laboratoria chipowe i detekcję fluorymetryczną. Przedstawione urządzenia opracowano w ramach europejskich i krajowych projektach naukowo-badawczych. Przedstawiono nową metodę i instrumentarium do detekcji fluorymetrycznej wykorzystujących powszechnie dostępne komponenty optoelektroniczne. Krótko opisano trzy przykłady demonstratorów wykorzystujących laboratoria chipowe w diagnostyce medycznej i weterynaryjnej wykorzystujące tę samą ideę odczytu fluorymetrycznego.

3

Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

Walczak R.

Bulletin of the Polish Academy of Sciences. Technical Sciences

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2011

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Vol. 59, nr 2

157-165

EN

The fluorometric detection in lab-on-a-chip devices for life-sciences is one of the main detection methods. Well know from “large-scale” analytical chemistry methodologies and instrumentation has been applied in lab-on-a-chip solutions. In most cases, optical instrumentation for fluorescence induction and detection is based on configuration and components borrowed from large laboratory instruments. As a result, optical instrumentation surrounding lab-on-a-chip is bulky, expensive and dedicated for operation only inside laboratory. In this paper a discussion on fluorescence detection in lab-on-a-chip is carried out. A novel image sensor – based detection instrumentation co-working with a “clever” software is described. Instrumentation is dedicated for operation in portable and low-cost devices for different life-science applications. Examples of applications of the novel method and instrumentation co-working with various lab-on-a-chips are presented on the base of author’s works.