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EN
Muscle relaxants and pain killers with their different types are widely used as combination approach for treatment of pain associated with several muscle spasm conditions. A sensitive and simple HPLC-UV detection method was developed in this work for simultaneous assay of Dantrolene (DNT) and co-administrated: Ibuprofen (IBU) and Diclofenac (DIC). After simple protein precipitation, separation was achieved using C₁₈ column (150 × 4.6 mm) with a mobile phase of acidified water with orthophosphoric acid (pH = 3.5) and acetonitrile using gradient elution with a flow rate of 1 mL/min. The DAD was adjusted at 380, 219, 280 and 240 nm to measure DNT, IBU, DIC, and dexamethasone (internal standard), respectively. Linearity was demonstrated over the range from 0.1 to 3 μg/mL, 1 to 40 μg/mL, and 0.1 to 2 μg/mL for DNT, IBU, and DIC, respectively. The validated method was applied successfully to compare the effect of co-administration of IBU or DIC on the pharmacokinetic profile of DNT.
EN
Low-temperature plasma production is possible as a result of photoionization using high-intensity extreme ultraviolet (EUV) and soft X-ray (SXR) pulses. Plasma of this type is also present in outer space, e.g., aurora borealis. It also occurs when high-velocity objects enter the atmosphere, during which period high temperatures can be produced locally by friction. Low-temperature plasma is also formed in an ambient gas surrounding the hot laser-produced plasma (LPP). In this work, a special system has been prepared for investigation of this type of plasma. The LPP was created inside a chamber fi lled with a gas under a low pressure, of the order of 1–50 mbar, by a laser pulse (3–9 J, 1–8 ns) focused onto a gas puff target. In such a case, the SXR/EUV radiation emitted from the LPP was partially absorbed in the low-density gas. In this case, high- and low-temperature plasmas (Te ~100 eV and ~1 eV, respectively) were created locally in the chamber. Investigation of the EUV-induced plasmas was performed mainly using spectral methods in ultraviolet/visible (UV/VIS) light. The measurements were performed using an echelle spectrometer, and additionally, spatial–temporal measurements were performed using an optical streak camera. Spectral analysis was supported by the PGOPHER numerical code.
EN
The Thomson parabola spectrometer (TPS) [1] is a well-known, universal diagnostic tool that is widely used in laser plasma experiments to measure the parameters of accelerated ions. In contrast to other popular ion diagnostics, such as semiconductor detectors or ion collectors, the TPS is not greatly affected by electromagnetic pulses generated during high-power laser interaction with matter and can be tuned to acquire data in various energy ranges of accelerated ions, depending on the goal of the experiment. Despite the many advantages of this diagnostic device, processing the collected data is a diffi cult task and requires a lot of caution during interpretation of gathered results. In this work, we introduce the basic principles of operation and data analysis based on the numerical tool created specifi cally for the TPS designed at the Institute of Plasma Physics and Laser Microfusion, present a range of data obtained during various recent experiments in which our TPS was used, and highlight the diffi culties in data analysis depending on the purpose of the experiment and the experimental setup.
EN
The influence of the slip factor on the MHD 2-liquid heat transfer flow of ionized gases within a channel between two non-conducting plates with Hall currents is investigated theoretically. Slip conditions were used to obtain solutions for the velocity and temperature fields, as well as the heat transfer rates. The flow characteristics of the two liquids are studied for estimates of the leading parameters, for instance the magnetic parameter, Hall and slip factors, viscosity, density, height, electrical conductivity and the thermal conductivity ratios. It was observed that an upsurge in temperature in the two zones is caused by the thermal conductivity proportion. The rate of heat transfer coefficient diminishes up to a certain point, after that it starts to increase as the magnetic and Hall parameters increase.
EN
It is proposed to use the Hall currents to model the transient magneto-hydrodynamic two liquid flows and heat transfer of ionized gases propelled by a common pressure gradient via a horizontal channel consisting of parallel porous plates. For the distributions of velocity and temperature, the principal partial differential equations that explain heat transfer flow under the chosen constraints are resolved. Graphical representations are given for the distributions of velocity, temperature, and heat transfer rates. This research will be carried out using non-conducting porous plate’s channel.
PL
Czystość powierzchni elementów ma istotne znaczenie w procesie produkcji. Kolejne etapy procesu obróbki, takie jak spawanie, klejenie, uszczelnianie, lakierowanie, wykonywanie nadruków czy lutowanie, wymagają wcześniej usunięcia zabrudzeń, w celu zapewnienia odpowiedniej jakości. Przemysłowe czyszczenie powierzchni z użyciem systemu Acerios, czyli opracowanej przez firmę Fronius technologii gorącej plazmy, wyróżnia się nieszkodliwością i efektywnością.
7
EN
Cutting is usually one of the initial and primary operations used when making welded structures and fabricating structural elements. Increasingly often, the preparation of elements involves the use of thermal, in particular plasma arc, cutting. The plasma arc cutting process consists in melting and ejecting liquid metal from the cut gap by highly concentrated plasma electric arc burning between a non-consumable electrode and a workpiece. The article presents results of tests concerning the influence of plasma gas on structural changes and chemical composition changes resulting from the cutting of unalloyed steel using air plasma arc. The tests revealed that the application of the air plasma arc cutting process led to the formation of an amorphous layer characterized by a very high nitrogen content (of approximately 1.6%) and a hardness of 750 HV 0.2. This high nitriding effect was triggered by the diffusion of nitrogen from the plasma gas. At the same time, the effect of air plasma arc gases on the liquid metal led to the carburising of the cut surface (up to approximately 0.5%) as well as to the burnout of alloying components (in accordance with the theory of selective oxidation of chemical elements). After the air plasma cutting process, the material structure revealed features intermediate between those of the structure formed through oxygen cutting and those of the structure formed as a result of nitrogen plasma cutting. The tests also revealed that the argon-hydrogen plasma cutting process had the lowest effect on the material subjected to cutting.
PL
Cięcie jest zwykle jedną z początkowych i podstawowych operacji procesu wytwarzania konstrukcji spawanych oraz wykonywania gotowych elementów konstrukcji. Coraz częściej do przygotowania elementów używa się cięcia termicznego, a w szczególności cięcia łukiem plazmowym. Proces cięcia łukiem plazmowym polega na stopieniu i wyrzuceniu ciekłego metalu ze szczeliny cięcia silnie skoncentrowanym plazmowym łukiem elektrycznym, jarzącym się między elektrodą nietopliwą a ciętym przedmiotem. W artykule przedstawiono wyniki badań wpływu gazu plazmowego na zmiany strukturalne i zmiany składu chemicznego powstałe w wyniku procesu cięcia łukiem plazmy z zastosowaniem powietrza w stali niestopowej. Wykazano, że w wyniku procesu cięcia plazmą z zastosowaniem powietrza na powierzchni cięcia powstaje warstwa bezpostaciowa o bardzo dużej zawartości azotu (około 1,6%) i twardości rzędu 750 HV 0,2. To silne naazotowanie jest spowodowane dyfuzją azotu z gazu plazmowego. Jednocześnie w wyniku oddziaływania gazów łuku plazmy z zastosowaniem powietrza na ciekły metal dochodzi do nawęglenia powierzchni cięcia (do około 0,5%) i wypalenia składników stopowych, w myśl teorii selektywnego utleniania pierwiastków chemicznych. Po procesie cięcia plazmą z zastosowaniem powietrza struktura materiału wykazuje cechy pośrednie między strukturą powstałą po procesie cięcia tlenem a plazmą z zastosowaniem azotu. Najmniejszy wpływ na materiał cięty wywiera proces cięcia plazmą z zastosowaniem mieszanki argon-wodór.
EN
Purpose: Development and validation of a selective analytical method to accurately and precisely quantify nicotine and cotinine levels in rat's plasma after exposure to tobacco cigarettes and tobacco water-pipe. Methods: An easy HPLC-Photodiode-Array Detection (PDA) method was developed and validated for simultaneous determination of nicotine and cotinine levels in plasma of 15 rats (10 rats after tobacco products exposure and 5 control rats). Nicotine and cotinine were extracted in one step from plasma using acetonitrile and concentrated to lowest volume using nitrogen stream. Results: The developed method offered a rapid analysis time of 14 min with single step of analytes extraction from rat's plasma with recovery percentage range between 93 and 95% and excellent linearity with correlation factor more than 0.994 with analytical range between 50 and 1000 ng mL⁻¹ and LOD of 25 ng mL⁻¹ and 23 ng mL⁻¹ for nicotine and cotinine, respectively. The analysis of rat's plasma after 28 days of exposure to tobacco cigarettes and tobacco water-pipe revealed that the average concentrations of 376 ng mL⁻¹ for cotinine and 223 ng mL⁻¹ for nicotine were obtained after tobacco cigarettes exposure, and 220 ng mL⁻¹ for cotinine and 192 ng mL⁻¹ for nicotine after tobacco water-pipe exposure. Conclusion: Higher nicotine and cotinine levels were found in plasma after tobacco cigarettes exposure than water-pipe exposure which may have potential undesirable effects on passive smokers in both cases.
PL
Problem spalania koksików powstających np. z niezupełnego spalania pyłu węglowego i/lub biomasy wymaga specjalnych warunków z powodu niskiej reaktywności koksików. Koksiki te charakteryzują się dużą zawartością substancji mineralnych i brakiem części lotnych, co między innymi powoduje, że mają wysoką temperaturę zapłonu i względnie stałą szybkość spalania w szerokim zakresie temperatur cząstek. Analiza termograwimetryczna dla różnych szybkości nagrzewu pokazała, że ze wzrostem szybkości nagrzewania do temperatury zapłonu jest możliwe uzyskanie zupełnego spalania. Takie warunki nagrzewu koksików gwarantuje zarówno plazma mikrofalowa jak i łukowa. Wstępne badania w reaktorze przepływowym potwierdziły oczekiwania co do stopnia wypalenia. Kolejno w pracy przedstawiono badania nad zastosowaniem palnika plazmowego do spalania koksików.
EN
The problem of minimizing high content of char in fly ash obtained under incomplete combustion of coal or biomass requires special conditions due to the low reactivity of coke. These coke are characterized by a high content of mineral substances and the low concentration of volatile matter, which means high ignition temperature and relatively constant combustion rate in a wide range of particle temperatures. Thermogravimetric analysis for different heating rates shows that with the increase of the heating rate to the ignition temperature, it is possible to obtain complete combustion of analysed fuel. Such conditions for heating the char are guaranteed by both microwave and arc plasma. Initial experimental tests in the flow reactor confirmed the expectations regarding the degree of burnout. In the paper research on the use of a plasma burner for coke is presented.
10
Content available remote Bonding methods of ceramic materials with deposited carbon layers
EN
In this paper, we present a proposed bonding technology of ceramic materials. It could be applied for manufacturing the excitation source for detection of elements by means of Optical Emission Spectroscopy (OES) method. The biggest challenge was choice of proper bonding technology for materials with screen printed carbon layers as an excitation electrodes. It was caused by a need to avoid a deterioration of electrical properties of the carbon layer during the bonding process. The bonding at low temperature with various glaze layers was verified with addition of noble elements and plasma modification. The results were promising, without any significant destruction of carbon layers.
PL
W tym artykule przedstawiamy proponowaną technologię łączenia materiałów ceramicznych która może być zastosowana przy wytwarzaniu źródła wzbudzenia do detekcji pierwiastków w metodzie optycznej spektroskopii emisyjnej. Wybór odpowiedniej technologii łączenia materiału z warstwami węglowymi wytwarzanymi metodą sitodruku był wyzwaniem z powodu konieczności uniknięcia degradacji właściwości elektrycznych węgla podczas procesu łączenia. Zweryfikowano łączenie w niskiej temperaturze z różnymi warstwami szkliwa z dodatkiem pierwiastków szlachetnych i modyfikacją plazmową. Wyniki były obiecujące, bez znacznego zniszczenia warstw węglowych.
EN
In this paper, based on a quantitative analysis of the parameters of plasma hardfacing of C45 steel with CastoMag 45554S wire, an optimisation of the hardfacing process parameters has been carried out. Experimental researches were carried out on the basis of an orthogonal plan, and the optimum hardfacing parameters, e.g.: intensity, voltage, wire feeding speed, were determined by the multiple regression method and the Taguchi method. It was found that the main current of the plasma arc had the greatest influence on the value of the fusion index. The highest S/N value was obtained for the following parameters: I = 120 A, U = 31 V, vn = 0.75 m/min, vd = 3.9 m/min, z = 12 mm.
PL
W oparciu o ilościową analizę parametrów napawania plazmowego stali C45 drutem CastoMag 45554S dokonano optymalizacji parametrów procesu napawania. Badania realizowano na podstawie planu ortogonalnego, a optymalne parametry napawania np.: natężenie, napięcie, prędkość podawania drutu zostały wyznaczone metodą regresji wielokrotnej oraz metodą Taguchi. Ustalono, że największy wpływ na wartość współczynnika wtopienia ma natężenie prądu głównego łuku plazmowego. Największą wartość współczynnika S/N uzyskano przy parametrach: I = 120 A, U = 31 V, vn = 0,75 m/min, vd = 3,9 m/min, z = 12 mm.
EN
An unsteady flow and heat transmission of ionized gases via a horizontal channel enclosed by non-conducting plates in a rotating framework with Hall currents is examined using electro-magnetohydrodynamic (EMHD) two-fluid heat flow. The Hall current impact is taken into account by assuming that the gases are totally ionized, the applied transverse magnetic field is very strong. For temperature and velocity distributions in two-fluid flow regions, the governing equations are solved analytically. For numerous physical parameters such as the Hartmann number, Hall parameter, rotation parameter, viscosity ratio, and so on, numerical solutions are visually displayed. It was discovered that an increase in temperature in the two regions is caused by the thermal conductivity ratio. It was also realized that an increase in rate of heat transfer coefficient at the plates is caused by either the Hartman number or the Hall parameter.
EN
Hall currents are used to investigate MHD unsteady two fluid flows and heat transport of plasma along a straight channel of conducting plates. In the two liquid zones, the velocity and temperature fields for the case of conducting side plates are obtained by solving the governing equations using a two-term series under the specified conditions. The distribution profiles are graphically resolved and examined. The distributions are thought to be dependent on the electron-to-total pressure ratio. The flow and heat transfer factors are also influenced by other parameters such as the Hartmann number, Hall parameter, rotation parameter, thermal conductivity and viscosity ratio.
EN
Although glimepiride (GLM) is the first-line treatment of Type II diabetes, low extraction recovery is still a significant limitation in previous plasma analysis methods. An optimized solid-phase extraction method of GLM in human plasma with excellent extraction recovery, 100 ± 0.06%, was achieved using liquid chromatography-electrospray ionization tandem mass spectrometry and Gliclazide (GLZ) as an internal standard. GLM was extracted from 100 µL plasma sample using Sep-Pak® vac 1cc (100 mg) C18 column and methylene chloride: methanol (2: 1, v/v) as eluant. Both GLM and GLZ were monitored by a triple quad mass spectrometer applying positive multiple reaction monitoring mode (+MRM). The protonated precursor ions and product ions of GLM and GLZ were m/z 491(352), and m/z 324 (127), respectively. The detection and measurement of low levels of GLM in human plasma reached to picogram range (limit of detection (LOD) = 60 pg/mL, limit of quantification (LOQ) = 200 pg/mL). The method was validated in terms of selectivity, linearity, recovery, accuracy, and precision. The method was successfully applied to the pharmacokinetic study of GLM following oral administration of 1 mg GLM tablets to 12 healthy volunteers.
EN
Tramadol, a strong pain killer known for its addictive problems is either co-administrated or co-formulated with other analgesics or muscle relaxants. The power of fluorescence detection in HPLC is tested to resolve such mixtures in plasma matrix to reach the required sensitivity with simple sample treatment using just protein precipitation. The aim of this work was to develop an eco-friendly and sensitive HPLC method with fluorimetric detection for analysis of Tramadol in its two binary mixtures with Ibuprofen (mixture 1) and Chlorzoxazone (mixture 2) in two combined dosage forms and spiked plasma. Separation was done using a C18 column with mobile phase of acetonitrile and water (pH 3.5) in gradient elution and 1 mL/min flow rate. Detection was carried out with λ excitation/λ emission of 220 and 307 nm, respectively. The method was applied to detect the two binary mixtures in real plasma samples after invivo application to rats, to assure that the drugs’ metabolites do not affect the sensitivity or selectivity of the assay. Evaluation of greenness of the proposed method was done using semi-quantitative Eco‐Scale and new Green Analytical Procedure Index which showed that this method can be a greener alternative with higher sensitivity for analysis of both mixtures. The method (15 min-assay) was linear over concentrations of 0.1–10 μg/mL and 0.1–33 μg/mL in plasma. In addition, the proposed method was validated per ICH as well as FDA bioanalytical methods’ validation guidelines.
EN
Triclabendazole is one of the main drugs used to treat liver fluke in livestock. A rapid LC-MS/MS method was developed and validated to determine ovine plasma levels of triclabendazole sulfoxide. A Gemini NX-C18 column was used to achieve analytical separation, with gradient elution of a mobile phase composed of 0.1% formic acid in acetonitril and 0.1% formic acid in water at flow rate of 0.6 mL/min. MRM with positive ESI ionization was used for the detection of triclabendazole sulfoxide (m/z 360.10 from m/z 376.97). Fenbendazole was used as internal standard. Plasma protein precipitation with acetonitrile was used for sample processing. The method was validated with regards to selectivity, linearity (r > 0.9939), within run and between run precision (CV < 8.9%) and accuracy (bias < 8.9%) over the concentration range 1–100 µg/mL plasma. The method developed is simple, selective and can be applied in bioequivalence and bioavailability studies.
PL
Materiały ceramiczne jak większość materiałów ulega procesowi utleniania się. Zjawisko to może być niezwykle niekorzystne z punktu widzenia wytrzymałości mechanicznej. Problem z utlenianiem szczególnie uciążliwy jest w przypadku nakładania powłok metodami plazmowymi, podczas których temperatura procesu jest bardzo wysoka co w naturalny sposób sprzyja utlenianiu się. W ramach badań postanowiono sprawdzić jak metoda natryskiwania plazmowego wpływa na właściwości nakładanej warstwy. Przebadano kilka metod natryskiwania, kontrolując utlenianie powłoki. W kolejnym kroku poszczególne powłoki poddawano badaniom diagnostycznym. Został określany stopień utleniania powłoki za pomocą rentgenowskiej analizy fazowej. Następnie przebadano właściwości mechaniczne otrzymanych powłok. Szczególną uwagę zwrócono na metodę natryskiwanie pod ciśnieniem atmosferycznym, z pierścieniową strugą ochronną. W ramach badań dokonano ukształtowania pierścieniowej strugi ochronnej zabezpieczającej przed utlenianiem materiał proszkowy, tworzący powłokę.
EN
Ceramic materials, like most materials, undergo the process of oxidation. This phenomenon can be extremely unfavorable from the point of view of mechanical strength. The problem with oxidation is particularly troublesome in the case of plasma coating, where the process temperature is very high, which naturally promotes oxidation. As part of the research, it was decided to check how the plasma spray method affects the properties of the applied layer. Several spraying methods have been tested to control the oxidation of the coating. In the next step, individual coatings were subjected to diagnostic tests. The degree of oxidation of the coating was determined by means of X-ray phase analysis. Then, the mechanical properties of the obtained coatings were tested. Particular attention was paid to the atmospheric pressure spraying method with an annular protective jet. In the research, an annular protective stream was shaped to protect the powder material forming the coating against oxidation.
EN
The submitted paper deals with the issue of change in structure, hardness and thermally affected zone of the samples of a toothed wheel part produced by technology of cutting by plasma, laser and water jet. The cutting process by laser and plasma technology causes intensive thermal affection of material and change in structure and microhardness in the cutting area referred to as thermally affected zone. The paper describes three material cutting technologies of the toothed wheel part. The experimental part contains description of experimental sample preparation, its hardness measurement and examination of structure along with further evaluation through graphs and photo documentation of structures.
EN
An electro-magnetohydrodynamic (EMHD) two fluid flow and heat transfer of ionized gases through a horizontal channel surrounded by non-conducting plates in a rotating framework with Hall currents is investigated. The Hall effect is considered with an assumption that the gases are completely ionized and the strength of the applied transverse magnetic field is strong. The governing equations are solved analytically for the temperature and velocity distributions in two fluid flow regions. The numerical solutions are demonstrated graphically for various physical parameters such the Hartmann number, Hall parameter, rotation parameter, and so on. It was noticed that an increment is either due to the Hall parameter or the rotation parameter reduces the temperature in the two regions.
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