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1 Journal of Ecological Engineering

1 2017

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Response of Soil Phosphatases to Three Different Ionic Liquids With Hexafluorophosphate Anion

Telesiński A., Śnioszek M., Biczak R., Pawłowska B.

Journal of Ecological Engineering

2017

Vol. 18, nr 2

86--91

The aim of this study was to determine the effect of three different ionic liquids (ILs): 1-butyl-1-methylpyrrolidynium hexafluorophosphate [Pyrrol][PF6], 1-butyl-1-methylpiperydynium hexafluorophosphate [Piper][PF6] and 1-butyl-4-methylopirydynium hexafluorophosphate [Piryd][PF6] on phosphatase activities in soil. The pot experiment was carried out on loamy sand (Corg content 9.0 g/kg) with spring barley as a tested plant. The ILs was used at the dosages of 0, 1, 10, 50, 100, 400, 700 and 1000 mg/kg dry matter (DM). On day 14, soil samples were collected and activities of alkaline phosphomonoesterase, acid phosphomonoesterase, phosphotriesterase and inorganic pyrophosphatase were determined. Based on the analysis of the effect measure η2 by variance analysis – ANOVA – the percentage shares of all variable factors affecting the activity of phosphatases were also defined. Obtained results showed that the application of different dosages of all ILs caused mainly non-significant changes in phosphatase activity. Changes in activity of phosphatases were often not dependent on IL dosages. The most sensitive for ILs were both phosphomonoesterases. The type of ILs had the highest percentage participation in formation of acid phosphomonoesterase activity, while dosages of ILs affected mainly alkaline phosphomonoesterase and phosphotriesterase activities.